{"id":{"repo_id":"maynooth","oai_identifier":"oai:mural.maynoothuniversity.ie:8850"},"canonical_url":"https://search.dev.ndltd.org/etd/maynooth/oai:mural.maynoothuniversity.ie:8850","repository":{"repo_id":"maynooth","name":"National University of Ireland - Maynooth","base_url":"http://mural.maynoothuniversity.ie/cgi/oai2"},"display":{"title":"Mathematical Modelling and Statistical Inference from Immune Response Data","abstract":"A hallmark of the adaptive immune response is the proliferation of pathogen-specific lymphocytes that leave in their wake a long lived population of cells that provide lasting immunity. A subject of debate is at which time point post infection those memory cells are produced during an adaptive immune response. In two ground-breaking studies, [Buchholz et al., 2013] and [Gerlach et al., 2013] introduced a new experimental method that allowed them to determine the number offspring from individual lymphocytes in vivo at a single harvesting time point. Through the development, application and fitting of a mathematical model, the authors of [Buchholz et al., 2013] concluded that memory cell precursors are produced before the effector cells that clear the original pathogen, contrary to prior understanding. Cohort level cell data in the paper [Kinjyo et al., 2015], however, challenges that deduction. In this thesis we sought to quantitatively reconcile these two reports by adopting the mathematical methodology of [Buchholz et al., 2013] to make it suitable for drawing inferences from the data in [Badovinac et al., 2007], [Schlub et al., 2010] and [Kinjyo et al., 2015]. When fitting to spleen and blood data reported in these papers, under the assumptions of the model, our conclusion is consistent with [Buchholz et al., 2013]: memory precursor cells appear before effector cells. However, an alternative possibility supported by the data in [Kinjyo et al., 2015] is that memory is created after the expansion phase, a deduction not possible from the data or mathematical methods in [Buchholz et al., 2013].","abstract_html":"A hallmark of the adaptive immune response is the proliferation of pathogen-specific lymphocytes that leave in their wake a long lived population of cells that provide lasting immunity. A subject of debate is at which time point post infection those memory cells are produced during an adaptive immune response. In two ground-breaking studies, [Buchholz et al., 2013] and [Gerlach et al., 2013] introduced a new experimental method that allowed them to determine the number offspring from individual lymphocytes in vivo at a single harvesting time point. Through the development, application and fitting of a mathematical model, the authors of [Buchholz et al., 2013] concluded that memory cell precursors are produced before the effector cells that clear the original pathogen, contrary to prior understanding. Cohort level cell data in the paper [Kinjyo et al., 2015], however, challenges that deduction. In this thesis we sought to quantitatively reconcile these two reports by adopting the mathematical methodology of [Buchholz et al., 2013] to make it suitable for drawing inferences from the data in [Badovinac et al., 2007], [Schlub et al., 2010] and [Kinjyo et al., 2015]. When fitting to spleen and blood data reported in these papers, under the assumptions of the model, our conclusion is consistent with [Buchholz et al., 2013]: memory precursor cells appear before effector cells. However, an alternative possibility supported by the data in [Kinjyo et al., 2015] is that memory is created after the expansion phase, a deduction not possible from the data or mathematical methods in [Buchholz et al., 2013].","abstract_has_math":false,"creators":["Miles, Alexander S."],"institution":"National University of Ireland Maynooth","degree_name":null,"degree_level":"masters","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017","date_published":"2017","updated_at":"2026-07-24T03:02:57Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Miles, Alexander S."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017"]},{"key":"dc:date.issued","label":"Date","values":["2017"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["National University of Ireland Maynooth"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://mural.maynoothuniversity.ie/id/eprint/8850/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["masters"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://mural.maynoothuniversity.ie/id/eprint/8850/1/AM%20-%20Thesis%20Submission%202.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A hallmark of the adaptive immune response is the proliferation of pathogen-specific lymphocytes that leave in their wake a long lived population of cells that provide lasting immunity. A subject of debate is at which time point post infection those memory cells are produced during an adaptive immune response. In two ground-breaking studies, [Buchholz et al., 2013] and [Gerlach et al., 2013] introduced a new experimental method that allowed them to determine the number offspring from individual lymphocytes in vivo at a single harvesting time point. Through the development, application and fitting of a mathematical model, the authors of [Buchholz et al., 2013] concluded that memory cell precursors are produced before the effector cells that clear the original pathogen, contrary to prior understanding. Cohort level cell data in the paper [Kinjyo et al., 2015], however, challenges that deduction. In this thesis we sought to quantitatively reconcile these two reports by adopting the mathematical methodology of [Buchholz et al., 2013] to make it suitable for drawing inferences from the data in [Badovinac et al., 2007], [Schlub et al., 2010] and [Kinjyo et al., 2015]. When fitting to spleen and blood data reported in these papers, under the assumptions of the model, our conclusion is consistent with [Buchholz et al., 2013]: memory precursor cells appear before effector cells. However, an alternative possibility supported by the data in [Kinjyo et al., 2015] is that memory is created after the expansion phase, a deduction not possible from the data or mathematical methods in [Buchholz et al., 2013]."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["Mathematical Modelling and Statistical Inference from Immune Response Data"]}]}],"canonical_facts":{"dc:creator":["Miles, Alexander S."],"dc:date":["2017"],"dc:date.issued":["2017"],"dc:description.abstract":["A hallmark of the adaptive immune response is the proliferation of pathogen-specific lymphocytes that leave in their wake a long lived population of cells that provide lasting immunity. A subject of debate is at which time point post infection those memory cells are produced during an adaptive immune response. In two ground-breaking studies, [Buchholz et al., 2013] and [Gerlach et al., 2013] introduced a new experimental method that allowed them to determine the number offspring from individual lymphocytes in vivo at a single harvesting time point. Through the development, application and fitting of a mathematical model, the authors of [Buchholz et al., 2013] concluded that memory cell precursors are produced before the effector cells that clear the original pathogen, contrary to prior understanding. Cohort level cell data in the paper [Kinjyo et al., 2015], however, challenges that deduction. In this thesis we sought to quantitatively reconcile these two reports by adopting the mathematical methodology of [Buchholz et al., 2013] to make it suitable for drawing inferences from the data in [Badovinac et al., 2007], [Schlub et al., 2010] and [Kinjyo et al., 2015]. When fitting to spleen and blood data reported in these papers, under the assumptions of the model, our conclusion is consistent with [Buchholz et al., 2013]: memory precursor cells appear before effector cells. However, an alternative possibility supported by the data in [Kinjyo et al., 2015] is that memory is created after the expansion phase, a deduction not possible from the data or mathematical methods in [Buchholz et al., 2013]."],"dc:format":["text"],"dc:identifier.uri":["https://mural.maynoothuniversity.ie/id/eprint/8850/1/AM%20-%20Thesis%20Submission%202.pdf"],"dc:language":["en"],"dc:publisher.institution":["National University of Ireland Maynooth"],"dc:relation.isreferencedby":["https://mural.maynoothuniversity.ie/id/eprint/8850/"],"dc:title":["Mathematical Modelling and Statistical Inference from Immune Response Data"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["masters"]},"updated_at":"2026-07-24T03:02:57Z"}