Studies on the novel detection and destruction of the enteroparasite Giardia lamblia and other veterinary problematic microorganisms examined under varying culture conditions
type: CreativeWork
description: Aims & Rationale: This study focuses on the hypothesis that every pet entering a hospital environment runs the potential risk of contracting a nosocomial infection. Giardia lamblia (G. lamblia) is a zoonotic unicellular flagellated protozoan parasite that infects both human and non-human mammals. The presence of pathogenic organisms in veterinary settings is a public health concern in relation to human and animal exposure. Veterinary clinics represent a significant risk factor for the zoonotic transfer of pathogens especially in cases of wound infection and the shedding of faecal matter. This study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means of disinfecting these parasites and biofilms. This study aims to breach the testing void for G. lamblia that currently exists in Ireland by using a combined in vitro HCT-8 cell culture-quantitative PCR assay for evaluating the efficacy of using pulsed UV light for treating G. lamblia parasites. This study aims to provide a source of data into novel disinfection mechanisms such as Pulsed UV light technologies as a potential clinical application in veterinary practice. Methods & Results: Biofilms were grown on veterinary relevant surfaces, i.e. stainless steel and PVC coupons using a CDC biofilm reactor and treated using Pulsed UV light. Pulsed light successfully inactivated all test species (Listeria, Salmonella, Bacillus, Escherichia) in planktonic and biofilm form with an increase in inactivation for every increase in UV dose. Biofilms were also used as part of a parasite entrapment study for this project. Giardia lamblia was seeded in the biofilm reactor and disinfection studies were carried out. In order to independently define inactivation during testing of the novel disinfection methods an in vitro cell culture model was adapted and used. A real time PCR assay was utilized to detect parasite DNA in Bacillus cereus biofilms on stainless steel and PVC…
abstract: Aims & Rationale: This study focuses on the hypothesis that every pet entering a hospital environment runs the potential risk of contracting a nosocomial infection. Giardia lamblia (G. lamblia) is a zoonotic unicellular flagellated protozoan parasite that infects both human and non-human mammals. The presence of pathogenic organisms in veterinary settings is a public health concern in relation to human and animal exposure. Veterinary clinics represent a significant risk factor for the zoonotic transfer of pathogens especially in cases of wound infection and the shedding of faecal matter. This study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means of disinfecting these parasites and biofilms. This study aims to breach the testing void for G. lamblia that currently exists in Ireland by using a combined in vitro HCT-8 cell culture-quantitative PCR assay for evaluating the efficacy of using pulsed UV light for treating G. lamblia parasites. This study aims to provide a source of data into novel disinfection mechanisms such as Pulsed UV light technologies as a potential clinical application in veterinary practice. Methods & Results: Biofilms were grown on veterinary relevant surfaces, i.e. stainless steel and PVC coupons using a CDC biofilm reactor and treated using Pulsed UV light. Pulsed light successfully inactivated all test species (Listeria, Salmonella, Bacillus, Escherichia) in planktonic and biofilm form with an increase in inactivation for every increase in UV dose. Biofilms were also used as part of a parasite entrapment study for this project. Giardia lamblia was seeded in the biofilm reactor and disinfection studies were carried out. In order to independently define inactivation during testing of the novel disinfection methods an in vitro cell culture model was adapted and used. A real time PCR assay was utilized to detect parasite DNA in Bacillus cereus biofilms on stainless steel and PVC…
author: Coughlan, Gillian
language: en
date published: 2015
date modified: 2026-07-24T03:02:54Z
provider: National University of Ireland - Maynooth
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: National University of Ireland Maynooth