{"id":{"repo_id":"manitoba","oai_identifier":"oai:mspace.lib.umanitoba.ca:1993/2602"},"canonical_url":"https://search.dev.ndltd.org/etd/manitoba/oai:mspace.lib.umanitoba.ca:1993/2602","repository":{"repo_id":"manitoba","name":"University of Manitoba","base_url":"https://mspace.lib.umanitoba.ca/oai/request"},"display":{"title":"The regulation of secondary metabolism from Tolypocladium inflatum, a study on strain improvement in cyclosporin A productivity and its relation to growth and glucose metabolism","abstract":"The fungal product cyclosporin A is a powerful immunosuppressive with antifungal activity produced as a secondary metabolite from ' Tolypocladium inflatum'. As its role in transplantation surgery increases, the demands on industry to improve yields intensifies. Mutants showing increased production are compared with strain, MS-1, the original strain for this work for glucose (dextrose) consumption and growth and a relationship with enzymes of the Embden-Meyerhof Pathway is investigated. Statistical analysis is performed to ensure significance. Classical methods of mutation and selection for strain improvement are utilized. Increased cyclosporin A-producing mutants display decreased glucose consumption and slower biomass build-up when compared to strain MS-1 (P < 0.001) suggesting a slower rate of growth supports higher production. Coarse control of the enzymes hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK) in cell-free extracts of 'T. inflatum' was investigated. Decreased activities of HK, PFK and PK activity was observed in higher cyclosporin A-producing strains with probabilities of significance of <0.01 for HK and PFK and <0.05 for PK. A peak of activity early in the fermentation was also observed with probabilities of <0.01 that change by day in enzyme activity is significant for HK, PFK and PK. This suggests a decreased role by the EM pathway and the early establishment of the biochemical route for glucose catabolism.","abstract_html":"The fungal product cyclosporin A is a powerful immunosuppressive with antifungal activity produced as a secondary metabolite from &#x27; Tolypocladium inflatum&#x27;. As its role in transplantation surgery increases, the demands on industry to improve yields intensifies. Mutants showing increased production are compared with strain, MS-1, the original strain for this work for glucose (dextrose) consumption and growth and a relationship with enzymes of the Embden-Meyerhof Pathway is investigated. Statistical analysis is performed to ensure significance. Classical methods of mutation and selection for strain improvement are utilized. Increased cyclosporin A-producing mutants display decreased glucose consumption and slower biomass build-up when compared to strain MS-1 (P &lt; 0.001) suggesting a slower rate of growth supports higher production. Coarse control of the enzymes hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK) in cell-free extracts of &#x27;T. inflatum&#x27; was investigated. Decreased activities of HK, PFK and PK activity was observed in higher cyclosporin A-producing strains with probabilities of significance of &lt;0.01 for HK and PFK and &lt;0.05 for PK. A peak of activity early in the fermentation was also observed with probabilities of &lt;0.01 that change by day in enzyme activity is significant for HK, PFK and PK. This suggests a decreased role by the EM pathway and the early establishment of the biochemical route for glucose catabolism.","abstract_has_math":false,"creators":["Swidinsky, Ken"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1998,"date_issued":"1998-07-01T00:00:00Z","date_published":"1998-07-01T00:00:00Z","updated_at":"2026-07-24T03:02:03Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1993/2602","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Swidinsky, Ken"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2007-07-12T17:50:30Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2007-07-12T17:50:30Z"]},{"key":"dc:date.issued","label":"Date","values":["1998-07-01T00:00:00Z"]},{"key":"dc:type","label":"Dc Type","values":["master thesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1993/2602"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The fungal product cyclosporin A is a powerful immunosuppressive with antifungal activity produced as a secondary metabolite from ' Tolypocladium inflatum'. As its role in transplantation surgery increases, the demands on industry to improve yields intensifies. Mutants showing increased production are compared with strain, MS-1, the original strain for this work for glucose (dextrose) consumption and growth and a relationship with enzymes of the Embden-Meyerhof Pathway is investigated. Statistical analysis is performed to ensure significance. Classical methods of mutation and selection for strain improvement are utilized. Increased cyclosporin A-producing mutants display decreased glucose consumption and slower biomass build-up when compared to strain MS-1 (P < 0.001) suggesting a slower rate of growth supports higher production. Coarse control of the enzymes hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK) in cell-free extracts of 'T. inflatum' was investigated. Decreased activities of HK, PFK and PK activity was observed in higher cyclosporin A-producing strains with probabilities of significance of <0.01 for HK and PFK and <0.05 for PK. A peak of activity early in the fermentation was also observed with probabilities of <0.01 that change by day in enzyme activity is significant for HK, PFK and PK. This suggests a decreased role by the EM pathway and the early establishment of the biochemical route for glucose catabolism."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf","text/plain"]},{"key":"dc:title","label":"Title","values":["The regulation of secondary metabolism from Tolypocladium inflatum, a study on strain improvement in cyclosporin A productivity and its relation to growth and glucose metabolism"]}]}],"canonical_facts":{"dc:creator":["Swidinsky, Ken"],"dc:date.accessioned":["2007-07-12T17:50:30Z"],"dc:date.available":["2007-07-12T17:50:30Z"],"dc:date.issued":["1998-07-01T00:00:00Z"],"dc:description.abstract":["The fungal product cyclosporin A is a powerful immunosuppressive with antifungal activity produced as a secondary metabolite from ' Tolypocladium inflatum'. As its role in transplantation surgery increases, the demands on industry to improve yields intensifies. Mutants showing increased production are compared with strain, MS-1, the original strain for this work for glucose (dextrose) consumption and growth and a relationship with enzymes of the Embden-Meyerhof Pathway is investigated. Statistical analysis is performed to ensure significance. Classical methods of mutation and selection for strain improvement are utilized. Increased cyclosporin A-producing mutants display decreased glucose consumption and slower biomass build-up when compared to strain MS-1 (P < 0.001) suggesting a slower rate of growth supports higher production. Coarse control of the enzymes hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK) in cell-free extracts of 'T. inflatum' was investigated. Decreased activities of HK, PFK and PK activity was observed in higher cyclosporin A-producing strains with probabilities of significance of <0.01 for HK and PFK and <0.05 for PK. A peak of activity early in the fermentation was also observed with probabilities of <0.01 that change by day in enzyme activity is significant for HK, PFK and PK. This suggests a decreased role by the EM pathway and the early establishment of the biochemical route for glucose catabolism."],"dc:format.mimetype":["application/pdf","text/plain"],"dc:identifier.uri":["http://hdl.handle.net/1993/2602"],"dc:language.iso":["eng"],"dc:title":["The regulation of secondary metabolism from Tolypocladium inflatum, a study on strain improvement in cyclosporin A productivity and its relation to growth and glucose metabolism"],"dc:type":["master thesis"]},"updated_at":"2026-07-24T03:02:03Z"}