Separation and Further Characterization of Hematopoietic Cell Populations Based on Phenotypic and Biophysical Properties
type: CreativeWork
description: Hematopoietic stem cells (HSC) are multipotent and possess self-renewal capacity. Thus, they can sustain a life-long hematopoiesis and are also capable of restoring the hematopoietic system when transplanted to a patient. In allogeneic hematopoietic stem cell transplantation (HSCT), a potent graft-versus-leukemia (GvL) effect must be balanced against the concomitant risk of severe immunological response to non-malignant host cells; graft-versus-host-disease (GvHD). In case of recipient-donor tissue antigen disparity, such as human leukocyte antigen (HLA)-mismatch or ABO-incompatibility, depletion of allo-reactive or incompatible blood constituents by HSCT graft processing may be required. The aim of this work was to investigate the development of human erythroid/myeloid progenitor cells and their progeny in normal differentiation and in the chimeric state of transfusion and allogeneic HSCT. Furthermore, novel technology platforms for optimal HSCT graft engineering in the setting of recipient-donor tissue antigen disparity were investigated. We utilized two in vitro culture systems (Paper II) to obtain cells of advanced neutrophil and erythroid maturation from adult human CD34+ bone marrow cells, and to study the relationship between clonogenicity, gene expression and phenotype during early myeloid development. Cells were sorted by fluorescence-activated-cell-sorting (FACS) according to selected surface markers into defined populations of sequential developmental stages for further analysis. Thus the expression profiles of several genes could to be directly correlated to changes in clonogenic potential and to lineage commitment, as defined by surface expression of CD15 (neutrophil differentiation) and group A antigen (eryhtroid differentiation). With regard to the established surface markers for neutrofil and erythroid lineage-affiliation, we investigated (Paper I) the origin of reappearing recipient-derived RBC in a patient with relapsed myelodysplastic syndrome (MD…
abstract: Hematopoietic stem cells (HSC) are multipotent and possess self-renewal capacity. Thus, they can sustain a life-long hematopoiesis and are also capable of restoring the hematopoietic system when transplanted to a patient. In allogeneic hematopoietic stem cell transplantation (HSCT), a potent graft-versus-leukemia (GvL) effect must be balanced against the concomitant risk of severe immunological response to non-malignant host cells; graft-versus-host-disease (GvHD). In case of recipient-donor tissue antigen disparity, such as human leukocyte antigen (HLA)-mismatch or ABO-incompatibility, depletion of allo-reactive or incompatible blood constituents by HSCT graft processing may be required. The aim of this work was to investigate the development of human erythroid/myeloid progenitor cells and their progeny in normal differentiation and in the chimeric state of transfusion and allogeneic HSCT. Furthermore, novel technology platforms for optimal HSCT graft engineering in the setting of recipient-donor tissue antigen disparity were investigated. We utilized two in vitro culture systems (Paper II) to obtain cells of advanced neutrophil and erythroid maturation from adult human CD34+ bone marrow cells, and to study the relationship between clonogenicity, gene expression and phenotype during early myeloid development. Cells were sorted by fluorescence-activated-cell-sorting (FACS) according to selected surface markers into defined populations of sequential developmental stages for further analysis. Thus the expression profiles of several genes could to be directly correlated to changes in clonogenic potential and to lineage commitment, as defined by surface expression of CD15 (neutrophil differentiation) and group A antigen (eryhtroid differentiation). With regard to the established surface markers for neutrofil and erythroid lineage-affiliation, we investigated (Paper I) the origin of reappearing recipient-derived RBC in a patient with relapsed myelodysplastic syndrome (MD…
author: Dykes, Josefina
language: eng
keywords: Hematology, Hematopoiesis, Blood Group, ABO-system, Hematopoietic Stem Cell Transplantation, Cell Separation
date published: 2013
date modified: 2026-07-24T02:59:49Z
provider: University of Lund
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Division of Hematology and Transfusion Medicine, Department of Laboratory Medicine, Lund University