{"id":{"repo_id":"lsu-thes","oai_identifier":"oai:repository.lsu.edu:gradschool_dissertations-1371"},"canonical_url":"https://search.dev.ndltd.org/etd/lsu-thes/oai:repository.lsu.edu:gradschool_dissertations-1371","repository":{"repo_id":"lsu-thes","name":"Lousiana State University","base_url":"https://repository.lsu.edu/do/oai/"},"display":{"title":"Receptor-mediated calcium entry in retinal amacrine cells","abstract":"In the vertebrate retina, multiple cell types express g protein-coupled receptors linked to phospholipase C. The signaling pathway engendered by activation of this enzyme can involve Ca<sup>2+</sup>-permeable transient receptor potential (TRP) channels. To begin to understand the role of these channels in the retina, we undertake an immunocytochemical localization of two TRPC channel subunits, TRPC1 and TRPC4. TRPC1 expression was observed in amacrine cells and their process in the chicken retina. TRPC4 expression was much more widespread with some degree of labeling found in all layers of the retina, and was shown to be expressed in Müller glial cells. Thus, the distributions of these two subunits indicate that different retinal cell types express TRPC channels containing different subunits. Recently, several sphingolipids have been demonstrated to play key roles in Ca<sup>2+</sup> mobilization in neurons. Sphingosine-1-phosphate is a sphingolipid metabolite that has been shown to activate a class of g protein-coupled receptors (S1PRs) in other cell types. In the present study, we examine the signaling properties of S1P in retinal amacrine cells. S1P produced a noisy, inward cation current in amacrine cells that occurred through activation of S1P1R and S1P3R. The S1P-induced current was PLC-sensitive and was eliminated with La<sup>3+</sup> and Gd<sup>2+</sup>, suggesting activation of TRPCs. S1P also elicited cytosolic Ca<sup>2+</sup> elevations. The S1P-induced Ca<sup>2+</sup> increase was mediated by S1P1R and S1P3R and was a result of both release of Ca<sup>2+</sup> from internal stores and Ca<sup>2+</sup> influx. Single-cell PCR amplification of TRPC channel subunits 1, 4, and 5 confirmed expression of these subunits in amacrine cells, suggesting that S1P is capable of activating TRPC-mediated Ca<sup>2+</sup> entry in retinal amacrine cells through a novel lipid signaling pathway.","abstract_html":"In the vertebrate retina, multiple cell types express g protein-coupled receptors linked to phospholipase C. The signaling pathway engendered by activation of this enzyme can involve Ca&lt;sup&gt;2+&lt;/sup&gt;-permeable transient receptor potential (TRP) channels. To begin to understand the role of these channels in the retina, we undertake an immunocytochemical localization of two TRPC channel subunits, TRPC1 and TRPC4. TRPC1 expression was observed in amacrine cells and their process in the chicken retina. TRPC4 expression was much more widespread with some degree of labeling found in all layers of the retina, and was shown to be expressed in Müller glial cells. Thus, the distributions of these two subunits indicate that different retinal cell types express TRPC channels containing different subunits. Recently, several sphingolipids have been demonstrated to play key roles in Ca&lt;sup&gt;2+&lt;/sup&gt; mobilization in neurons. Sphingosine-1-phosphate is a sphingolipid metabolite that has been shown to activate a class of g protein-coupled receptors (S1PRs) in other cell types. In the present study, we examine the signaling properties of S1P in retinal amacrine cells. S1P produced a noisy, inward cation current in amacrine cells that occurred through activation of S1P1R and S1P3R. The S1P-induced current was PLC-sensitive and was eliminated with La&lt;sup&gt;3+&lt;/sup&gt; and Gd&lt;sup&gt;2+&lt;/sup&gt;, suggesting activation of TRPCs. S1P also elicited cytosolic Ca&lt;sup&gt;2+&lt;/sup&gt; elevations. The S1P-induced Ca&lt;sup&gt;2+&lt;/sup&gt; increase was mediated by S1P1R and S1P3R and was a result of both release of Ca&lt;sup&gt;2+&lt;/sup&gt; from internal stores and Ca&lt;sup&gt;2+&lt;/sup&gt; influx. Single-cell PCR amplification of TRPC channel subunits 1, 4, and 5 confirmed expression of these subunits in amacrine cells, suggesting that S1P is capable of activating TRPC-mediated Ca&lt;sup&gt;2+&lt;/sup&gt; entry in retinal amacrine cells through a novel lipid signaling pathway.","abstract_has_math":false,"creators":["Crousillac, Scott Michael"],"institution":"Biological Sciences","degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007-01-01T08:00:00Z","date_published":"2007-01-01T08:00:00Z","updated_at":"2026-07-24T02:57:30Z","subjects":["store-operated channels","sphingosine-1-phosphate","trpc"],"languages":[],"rights":["unrestricted","Release the entire work immediately for access worldwide."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["etd-11132007-094713","https://repository.lsu.edu/gradschool_dissertations/372"],"render_values":[{"text":"etd-11132007-094713","href":null,"code":true},{"text":"https://repository.lsu.edu/gradschool_dissertations/372","href":"https://repository.lsu.edu/gradschool_dissertations/372","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.31390/gradschool_dissertations.372","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Crousillac, Scott Michael"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2007-10-05"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2022-05-12T23:08:36Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Biological Sciences"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["store-operated channels","sphingosine-1-phosphate","trpc"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["unrestricted","Release the entire work immediately for access worldwide."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["etd-11132007-094713","10.31390/gradschool_dissertations.372","https://repository.lsu.edu/gradschool_dissertations/372"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In the vertebrate retina, multiple cell types express g protein-coupled receptors linked to phospholipase C. The signaling pathway engendered by activation of this enzyme can involve Ca<sup>2+</sup>-permeable transient receptor potential (TRP) channels. To begin to understand the role of these channels in the retina, we undertake an immunocytochemical localization of two TRPC channel subunits, TRPC1 and TRPC4. TRPC1 expression was observed in amacrine cells and their process in the chicken retina. TRPC4 expression was much more widespread with some degree of labeling found in all layers of the retina, and was shown to be expressed in Müller glial cells. Thus, the distributions of these two subunits indicate that different retinal cell types express TRPC channels containing different subunits. Recently, several sphingolipids have been demonstrated to play key roles in Ca<sup>2+</sup> mobilization in neurons. Sphingosine-1-phosphate is a sphingolipid metabolite that has been shown to activate a class of g protein-coupled receptors (S1PRs) in other cell types. In the present study, we examine the signaling properties of S1P in retinal amacrine cells. S1P produced a noisy, inward cation current in amacrine cells that occurred through activation of S1P1R and S1P3R. The S1P-induced current was PLC-sensitive and was eliminated with La<sup>3+</sup> and Gd<sup>2+</sup>, suggesting activation of TRPCs. S1P also elicited cytosolic Ca<sup>2+</sup> elevations. The S1P-induced Ca<sup>2+</sup> increase was mediated by S1P1R and S1P3R and was a result of both release of Ca<sup>2+</sup> from internal stores and Ca<sup>2+</sup> influx. Single-cell PCR amplification of TRPC channel subunits 1, 4, and 5 confirmed expression of these subunits in amacrine cells, suggesting that S1P is capable of activating TRPC-mediated Ca<sup>2+</sup> entry in retinal amacrine cells through a novel lipid signaling pathway."]},{"key":"dc:title","label":"Title","values":["Receptor-mediated calcium entry in retinal amacrine cells"]}]}],"canonical_facts":{"dc:creator":["Crousillac, Scott Michael"],"dc:date":["2007-10-05"],"dc:date.available":["2022-05-12T23:08:36Z"],"dc:description.abstract":["In the vertebrate retina, multiple cell types express g protein-coupled receptors linked to phospholipase C. The signaling pathway engendered by activation of this enzyme can involve Ca<sup>2+</sup>-permeable transient receptor potential (TRP) channels. To begin to understand the role of these channels in the retina, we undertake an immunocytochemical localization of two TRPC channel subunits, TRPC1 and TRPC4. TRPC1 expression was observed in amacrine cells and their process in the chicken retina. TRPC4 expression was much more widespread with some degree of labeling found in all layers of the retina, and was shown to be expressed in Müller glial cells. Thus, the distributions of these two subunits indicate that different retinal cell types express TRPC channels containing different subunits. Recently, several sphingolipids have been demonstrated to play key roles in Ca<sup>2+</sup> mobilization in neurons. Sphingosine-1-phosphate is a sphingolipid metabolite that has been shown to activate a class of g protein-coupled receptors (S1PRs) in other cell types. In the present study, we examine the signaling properties of S1P in retinal amacrine cells. S1P produced a noisy, inward cation current in amacrine cells that occurred through activation of S1P1R and S1P3R. The S1P-induced current was PLC-sensitive and was eliminated with La<sup>3+</sup> and Gd<sup>2+</sup>, suggesting activation of TRPCs. S1P also elicited cytosolic Ca<sup>2+</sup> elevations. The S1P-induced Ca<sup>2+</sup> increase was mediated by S1P1R and S1P3R and was a result of both release of Ca<sup>2+</sup> from internal stores and Ca<sup>2+</sup> influx. Single-cell PCR amplification of TRPC channel subunits 1, 4, and 5 confirmed expression of these subunits in amacrine cells, suggesting that S1P is capable of activating TRPC-mediated Ca<sup>2+</sup> entry in retinal amacrine cells through a novel lipid signaling pathway."],"dc:identifier":["etd-11132007-094713","10.31390/gradschool_dissertations.372","https://repository.lsu.edu/gradschool_dissertations/372"],"dc:rights":["unrestricted","Release the entire work immediately for access worldwide."],"dc:subject":["store-operated channels","sphingosine-1-phosphate","trpc"],"dc:title":["Receptor-mediated calcium entry in retinal amacrine cells"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"],"thesis:institution_name":["Biological Sciences"]},"updated_at":"2026-07-24T02:57:30Z"}