Antigen stimulation of T lymphocytes induces the activation of phospholipase D (PLD) signaling. Phospholipase D (PLD) is a phosphodiesterase that catalyzes the conversion of phosphatidyl choline (PC) to phosphatidic acid (PA). PA is an important lipid second messenger and is known to mediate a variety of cellular functions. However, the specific role of PA in T lymphocytes has not been established. Previous studies indicated differential requirement for TCR induced PLD signaling in regulatory and non-regulatory T cells. Inhibition of TCR induced PLD signal preferentially suppressed the growth of non-regulatory T cells while allowing the proliferation of regulatory T cells in the presence of exogenous IL-2. Based on this observation, we hypothesized that PLD signaling is a critical factor that balances the population dynamics between regulatory and non-regulatory T cells.
For my dissertation work, I focused on elucidating the functional and molecular regulation of PLD signaling in T cells. In this study, we identified that various exogenous (alcohol and
PLD has two isoforms namely PLD1 and PLD2 expressed in mammalian cells. These isoforms are 50% identical and have distinct localization in the cell. PLD1 has peri-nuclear localization while PLD2 localizes to the plasma membrane. Molecular analysis of PLD1 and PLD2 using domain deletion suggested that a region unique to PLD1 known as the `loop' confers the distinct peri-nuclear localization of PLD1.
Further, we addressed the specific function of PLD2 in CD4 T cells using PLD2 knock out mice. We found that PLD2 is dispensable for T cell activation and proliferation but might play an important role in effector T cell differentiation. The results from this study delineate some of the functions of PLD signaling in T cells and provide insight into immunological regulation during T cell activation.
","abstract_html":"<p>Antigen stimulation of T lymphocytes induces the activation of phospholipase D (PLD) signaling. Phospholipase D (PLD) is a phosphodiesterase that catalyzes the conversion of phosphatidyl choline (PC) to phosphatidic acid (PA). PA is an important lipid second messenger and is known to mediate a variety of cellular functions. However, the specific role of PA in T lymphocytes has not been established. Previous studies indicated differential requirement for TCR induced PLD signaling in regulatory and non-regulatory T cells. Inhibition of TCR induced PLD signal preferentially suppressed the growth of non-regulatory T cells while allowing the proliferation of regulatory T cells in the presence of exogenous IL-2. Based on this observation, we hypothesized that PLD signaling is a critical factor that balances the population dynamics between regulatory and non-regulatory T cells.</p><p>For my dissertation work, I focused on elucidating the functional and molecular regulation of PLD signaling in T cells. In this study, we identified that various exogenous (alcohol and <italic>Clostridium difficile</italic> toxin) and endogenous factors (adenosine) modulate PLD signaling altering the population dynamics of regulatory and non-regulatory T cells.</p><p>PLD has two isoforms namely PLD1 and PLD2 expressed in mammalian cells. These isoforms are 50% identical and have distinct localization in the cell. PLD1 has peri-nuclear localization while PLD2 localizes to the plasma membrane. Molecular analysis of PLD1 and PLD2 using domain deletion suggested that a region unique to PLD1 known as the `loop' confers the distinct peri-nuclear localization of PLD1.</p><p>Further, we addressed the specific function of PLD2 in CD4 T cells using PLD2 knock out mice. We found that PLD2 is dispensable for T cell activation and proliferation but might play an important role in effector T cell differentiation. The results from this study delineate some of the functions of PLD signaling in T cells and provide insight into immunological regulation during T cell activation.</p>","abstract_has_math":false,"creators":["Chandrasekaran, Uma"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation","degree_discipline":"Microbiology and Immunology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-01-01T08:00:00Z","date_published":"2010-01-01T08:00:00Z","updated_at":"2026-07-24T02:55:08Z","subjects":["CD4 T CELLS","EFFECTOR T CELLS","PLD SIGNALING","REGULATORY T CELLS","Immunology and Infectious Disease"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://ecommons.luc.edu/luc_diss/162","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Chandrasekaran, Uma"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2016-03-31T17:08:32Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology and Immunology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["CD4 T CELLS","EFFECTOR T CELLS","PLD SIGNALING","REGULATORY T CELLS","Immunology and Infectious Disease"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://ecommons.luc.edu/luc_diss/162"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Antigen stimulation of T lymphocytes induces the activation of phospholipase D (PLD) signaling. Phospholipase D (PLD) is a phosphodiesterase that catalyzes the conversion of phosphatidyl choline (PC) to phosphatidic acid (PA). PA is an important lipid second messenger and is known to mediate a variety of cellular functions. However, the specific role of PA in T lymphocytes has not been established. Previous studies indicated differential requirement for TCR induced PLD signaling in regulatory and non-regulatory T cells. Inhibition of TCR induced PLD signal preferentially suppressed the growth of non-regulatory T cells while allowing the proliferation of regulatory T cells in the presence of exogenous IL-2. Based on this observation, we hypothesized that PLD signaling is a critical factor that balances the population dynamics between regulatory and non-regulatory T cells.
For my dissertation work, I focused on elucidating the functional and molecular regulation of PLD signaling in T cells. In this study, we identified that various exogenous (alcohol and
PLD has two isoforms namely PLD1 and PLD2 expressed in mammalian cells. These isoforms are 50% identical and have distinct localization in the cell. PLD1 has peri-nuclear localization while PLD2 localizes to the plasma membrane. Molecular analysis of PLD1 and PLD2 using domain deletion suggested that a region unique to PLD1 known as the `loop' confers the distinct peri-nuclear localization of PLD1.
Further, we addressed the specific function of PLD2 in CD4 T cells using PLD2 knock out mice. We found that PLD2 is dispensable for T cell activation and proliferation but might play an important role in effector T cell differentiation. The results from this study delineate some of the functions of PLD signaling in T cells and provide insight into immunological regulation during T cell activation.
"]},{"key":"dc:title","label":"Title","values":["Phospholipase D Signaling in T Cells"]}]}],"canonical_facts":{"dc:creator":["Chandrasekaran, Uma"],"dc:date.available":["2016-03-31T17:08:32Z"],"dc:description.abstract":["Antigen stimulation of T lymphocytes induces the activation of phospholipase D (PLD) signaling. Phospholipase D (PLD) is a phosphodiesterase that catalyzes the conversion of phosphatidyl choline (PC) to phosphatidic acid (PA). PA is an important lipid second messenger and is known to mediate a variety of cellular functions. However, the specific role of PA in T lymphocytes has not been established. Previous studies indicated differential requirement for TCR induced PLD signaling in regulatory and non-regulatory T cells. Inhibition of TCR induced PLD signal preferentially suppressed the growth of non-regulatory T cells while allowing the proliferation of regulatory T cells in the presence of exogenous IL-2. Based on this observation, we hypothesized that PLD signaling is a critical factor that balances the population dynamics between regulatory and non-regulatory T cells.
For my dissertation work, I focused on elucidating the functional and molecular regulation of PLD signaling in T cells. In this study, we identified that various exogenous (alcohol and
PLD has two isoforms namely PLD1 and PLD2 expressed in mammalian cells. These isoforms are 50% identical and have distinct localization in the cell. PLD1 has peri-nuclear localization while PLD2 localizes to the plasma membrane. Molecular analysis of PLD1 and PLD2 using domain deletion suggested that a region unique to PLD1 known as the `loop' confers the distinct peri-nuclear localization of PLD1.
Further, we addressed the specific function of PLD2 in CD4 T cells using PLD2 knock out mice. We found that PLD2 is dispensable for T cell activation and proliferation but might play an important role in effector T cell differentiation. The results from this study delineate some of the functions of PLD signaling in T cells and provide insight into immunological regulation during T cell activation.
"],"dc:identifier":["https://ecommons.luc.edu/luc_diss/162"],"dc:subject":["CD4 T CELLS","EFFECTOR T CELLS","PLD SIGNALING","REGULATORY T CELLS","Immunology and Infectious Disease"],"dc:title":["Phospholipase D Signaling in T Cells"],"thesis:degree_discipline":["Microbiology and Immunology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T02:55:08Z"}