{"id":{"repo_id":"london-metro","oai_identifier":"oai:repository.londonmet.ac.uk:7470"},"canonical_url":"https://search.dev.ndltd.org/etd/london-metro/oai:repository.londonmet.ac.uk:7470","repository":{"repo_id":"london-metro","name":"London Metropolitan University","base_url":"https://repository.londonmet.ac.uk/cgi/oai2"},"display":{"title":"Regulation of n-3 PUFA biosynthesis in proliferating and differentiated PC12 pheochromocytoma cells","abstract":"Regulation of polyunsaturated fatty acid (PUFA) biosynthesis in proliferating and NGF-differentiated PC12 pheochromocytoma cells deficient in n-3 docosahexaenoic acid (DHA, 22:6n- 3) was studied. A dose- and time dependent increase in eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3) and DHA in phosphatidylethanolamine (EPG) and phosphatidylserine (SPG) glycerophospholipids (GPL) via the elongation/desaturation pathway following alpha-linolenic acid (ALA, 18:3n-3) supplements was observed.That was accompanied by a marked reduction of eicosatrienoic acid (Mead acid, 20:3n-9), an index of PUFA deficiency. EPA supplements were similarly converted to 22:5n-3 and 22:6n-3. On the other hand, supplements of linoleic acid (LA, 18:2n-6) were not effectively converted into higher n-6 PUFA intermediates nor did they impair elongation/desaturation of ALA. A marked decrease in the newly synthesized 22:5n-3 and 22:6n-3 following ALA or EPA supplements was observed after nerve growth factor (NGF)-induced differentiation. NGF also inhibited the last step in 22:5n-6 formation from LA. NGF alters differently the expression of several genes involved in the FA metabolism; ElovJ5, EJovJ2, Fads2 and Ppara genes encoding, respectively, for ELOVL5 elongase, ELOVL2 elongase, Delta6 desaturase (D6D) and the nuclear transcription factor PPARa. LA up-regulates the expression of the above genes, whereas ALA specifically up-regUlates the mRNA levels of Fads2 and Elovl5. The results found in this thesis emphasise the importance of overcoming n-3 PUFA deficiency, and raise the possibility that growth factor regulation of the last step in PUFA biosynthesis may constitute an important feature of neuronal phenotype acquisition.","abstract_html":"Regulation of polyunsaturated fatty acid (PUFA) biosynthesis in proliferating and NGF-differentiated PC12 pheochromocytoma cells deficient in n-3 docosahexaenoic acid (DHA, 22:6n- 3) was studied. A dose- and time dependent increase in eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3) and DHA in phosphatidylethanolamine (EPG) and phosphatidylserine (SPG) glycerophospholipids (GPL) via the elongation/desaturation pathway following alpha-linolenic acid (ALA, 18:3n-3) supplements was observed.That was accompanied by a marked reduction of eicosatrienoic acid (Mead acid, 20:3n-9), an index of PUFA deficiency. EPA supplements were similarly converted to 22:5n-3 and 22:6n-3. On the other hand, supplements of linoleic acid (LA, 18:2n-6) were not effectively converted into higher n-6 PUFA intermediates nor did they impair elongation/desaturation of ALA. A marked decrease in the newly synthesized 22:5n-3 and 22:6n-3 following ALA or EPA supplements was observed after nerve growth factor (NGF)-induced differentiation. NGF also inhibited the last step in 22:5n-6 formation from LA. NGF alters differently the expression of several genes involved in the FA metabolism; ElovJ5, EJovJ2, Fads2 and Ppara genes encoding, respectively, for ELOVL5 elongase, ELOVL2 elongase, Delta6 desaturase (D6D) and the nuclear transcription factor PPARa. LA up-regulates the expression of the above genes, whereas ALA specifically up-regUlates the mRNA levels of Fads2 and Elovl5. The results found in this thesis emphasise the importance of overcoming n-3 PUFA deficiency, and raise the possibility that growth factor regulation of the last step in PUFA biosynthesis may constitute an important feature of neuronal phenotype acquisition.","abstract_has_math":false,"creators":["Msika, Ora"],"institution":"London Metropolitan University","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012","date_published":"2012","updated_at":"2026-07-24T02:54:38Z","subjects":["610 Medicine & health"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.grantnumber","label":"Dc Identifier Grantnumber","values":["N/A"],"render_values":[{"text":"N/A","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.sponsor","label":"Sponsor","values":["London Metropolitan University"]},{"key":"dc:creator","label":"Author","values":["Msika, Ora"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2012"]},{"key":"dc:date.issued","label":"Date","values":["2012"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Human Sciences (SHSC)","Faculty of Life Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["London Metropolitan University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://repository.londonmet.ac.uk/7470/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["610 Medicine & health"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.grantnumber","label":"Dc Identifier Grantnumber","values":["N/A"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://repository.londonmet.ac.uk/7470/1/590148.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Regulation of polyunsaturated fatty acid (PUFA) biosynthesis in proliferating and NGF-differentiated PC12 pheochromocytoma cells deficient in n-3 docosahexaenoic acid (DHA, 22:6n- 3) was studied. A dose- and time dependent increase in eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3) and DHA in phosphatidylethanolamine (EPG) and phosphatidylserine (SPG) glycerophospholipids (GPL) via the elongation/desaturation pathway following alpha-linolenic acid (ALA, 18:3n-3) supplements was observed.That was accompanied by a marked reduction of eicosatrienoic acid (Mead acid, 20:3n-9), an index of PUFA deficiency. EPA supplements were similarly converted to 22:5n-3 and 22:6n-3. On the other hand, supplements of linoleic acid (LA, 18:2n-6) were not effectively converted into higher n-6 PUFA intermediates nor did they impair elongation/desaturation of ALA. A marked decrease in the newly synthesized 22:5n-3 and 22:6n-3 following ALA or EPA supplements was observed after nerve growth factor (NGF)-induced differentiation. NGF also inhibited the last step in 22:5n-6 formation from LA. NGF alters differently the expression of several genes involved in the FA metabolism; ElovJ5, EJovJ2, Fads2 and Ppara genes encoding, respectively, for ELOVL5 elongase, ELOVL2 elongase, Delta6 desaturase (D6D) and the nuclear transcription factor PPARa. LA up-regulates the expression of the above genes, whereas ALA specifically up-regUlates the mRNA levels of Fads2 and Elovl5. The results found in this thesis emphasise the importance of overcoming n-3 PUFA deficiency, and raise the possibility that growth factor regulation of the last step in PUFA biosynthesis may constitute an important feature of neuronal phenotype acquisition."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["Regulation of n-3 PUFA biosynthesis in proliferating and differentiated PC12 pheochromocytoma cells"]}]}],"canonical_facts":{"dc:contributor.sponsor":["London Metropolitan University"],"dc:creator":["Msika, Ora"],"dc:date":["2012"],"dc:date.issued":["2012"],"dc:description.abstract":["Regulation of polyunsaturated fatty acid (PUFA) biosynthesis in proliferating and NGF-differentiated PC12 pheochromocytoma cells deficient in n-3 docosahexaenoic acid (DHA, 22:6n- 3) was studied. A dose- and time dependent increase in eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3) and DHA in phosphatidylethanolamine (EPG) and phosphatidylserine (SPG) glycerophospholipids (GPL) via the elongation/desaturation pathway following alpha-linolenic acid (ALA, 18:3n-3) supplements was observed.That was accompanied by a marked reduction of eicosatrienoic acid (Mead acid, 20:3n-9), an index of PUFA deficiency. EPA supplements were similarly converted to 22:5n-3 and 22:6n-3. On the other hand, supplements of linoleic acid (LA, 18:2n-6) were not effectively converted into higher n-6 PUFA intermediates nor did they impair elongation/desaturation of ALA. A marked decrease in the newly synthesized 22:5n-3 and 22:6n-3 following ALA or EPA supplements was observed after nerve growth factor (NGF)-induced differentiation. NGF also inhibited the last step in 22:5n-6 formation from LA. NGF alters differently the expression of several genes involved in the FA metabolism; ElovJ5, EJovJ2, Fads2 and Ppara genes encoding, respectively, for ELOVL5 elongase, ELOVL2 elongase, Delta6 desaturase (D6D) and the nuclear transcription factor PPARa. LA up-regulates the expression of the above genes, whereas ALA specifically up-regUlates the mRNA levels of Fads2 and Elovl5. The results found in this thesis emphasise the importance of overcoming n-3 PUFA deficiency, and raise the possibility that growth factor regulation of the last step in PUFA biosynthesis may constitute an important feature of neuronal phenotype acquisition."],"dc:format":["text"],"dc:identifier.grantnumber":["N/A"],"dc:identifier.uri":["https://repository.londonmet.ac.uk/7470/1/590148.pdf"],"dc:publisher.department":["School of Human Sciences (SHSC)","Faculty of Life Sciences"],"dc:publisher.institution":["London Metropolitan University"],"dc:relation.isreferencedby":["https://repository.londonmet.ac.uk/7470/"],"dc:subject":["610 Medicine & health"],"dc:title":["Regulation of n-3 PUFA biosynthesis in proliferating and differentiated PC12 pheochromocytoma cells"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T02:54:38Z"}