Isolation and characterisation of anti-diabetic pharmacological activities of phytoestrogens and components of Moringa peregrina (Forssk) Fiori
type: CreativeWork
description: Diabetes is an endocrine disease characterised by a chronic increase in blood sugar levels caused by a deficiency of insulin production, which leads to type 1 diabetes, or by a loss of tissue response to insulin, which leads to type 2 diabetes. The disease leads to disruption of metabolism, vascular damage and damage to the nervous system, as well as damage to other organs and systems. Type 2 diabetes is becoming more common throughout the world, due to poor nutrition and lifestyle, and genetic background. Efforts have accordingly been increased towards developing and refining treatments as well as to addressing the underlying causes of the disease. Many parts of the world have a documented history of the use of plants to treat diabetes, and these can be an attractive, local, alternative to expensive pharmaceutical medicines. Accordingly there is an increasing interest in identifying new phytochemicals with proven pharmacological effects on diabetes. The tree Moringa peregrina is commonly found throughout the Middle East and the oil from its seeds has been used for thousands of years. Other members of the Moringa family have recorded anti-diabetic family and Moringa peregrina was chosen for investigation in this work with the aim of characterising anti-diabetic activity from its leaves. Six extracts were prepared using solvents water, methanol, butanol, ethyl acetate, chloroform and hexane, based on standard extraction techniques. The study was designed to assess the effect of these six extracts on the uptake of glucose in a human hepatoma cell line (HepG2) using a well-studied fluorescent derivative of glucose, 2-2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino glucose (2-NBDG). The cells can take up 2-NBDG instead of glucose via glucose transporters and its uptake is indicative of the capacity of cells to take up glucose, i.e. of the number of active glucose transporters on the cell surface. The results revealed that the extracts prepared with ethyl acetate and chl…
abstract: Diabetes is an endocrine disease characterised by a chronic increase in blood sugar levels caused by a deficiency of insulin production, which leads to type 1 diabetes, or by a loss of tissue response to insulin, which leads to type 2 diabetes. The disease leads to disruption of metabolism, vascular damage and damage to the nervous system, as well as damage to other organs and systems. Type 2 diabetes is becoming more common throughout the world, due to poor nutrition and lifestyle, and genetic background. Efforts have accordingly been increased towards developing and refining treatments as well as to addressing the underlying causes of the disease. Many parts of the world have a documented history of the use of plants to treat diabetes, and these can be an attractive, local, alternative to expensive pharmaceutical medicines. Accordingly there is an increasing interest in identifying new phytochemicals with proven pharmacological effects on diabetes. The tree Moringa peregrina is commonly found throughout the Middle East and the oil from its seeds has been used for thousands of years. Other members of the Moringa family have recorded anti-diabetic family and Moringa peregrina was chosen for investigation in this work with the aim of characterising anti-diabetic activity from its leaves. Six extracts were prepared using solvents water, methanol, butanol, ethyl acetate, chloroform and hexane, based on standard extraction techniques. The study was designed to assess the effect of these six extracts on the uptake of glucose in a human hepatoma cell line (HepG2) using a well-studied fluorescent derivative of glucose, 2-2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino glucose (2-NBDG). The cells can take up 2-NBDG instead of glucose via glucose transporters and its uptake is indicative of the capacity of cells to take up glucose, i.e. of the number of active glucose transporters on the cell surface. The results revealed that the extracts prepared with ethyl acetate and chl…
author: Roostaei, Davoud
keywords: 580 Plants (Botany), 610 Medicine & health
date published: 2016
date modified: 2026-07-24T02:54:23Z
provider: London Metropolitan University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: London Metropolitan University