This thesis describes an in vivo perfusion apparatus which can continuously deliver controlled quantities of solutions to localized, contralateral intra oral areas in albino rats. Three animals were per fused on the experimental area (the mesial sulcular area of the maxillary first molar) on one side with tap water and trypan blue. Localization of the solution delivered and minimal histological changes due to the presence of the apparatus was demonstrated on these animals. A 1% solution of trypsin was perfused on the experimental area of one animal for one day at the rate of 4.8 ml/day and on another animal for four days at the same rate. A 1% pronase solution was perfused for four days on one animal at the rate of .96 ml/day and another animal at 4.8 ml/day. The appropriate control solutions were delivered on the contralateral side of each animal undergoing enzyme perfusion. Data obtained indicated moderate inflammatory changes were noted on the side receiving the enzyme. More meaningful results should be obtained with the modifications discussed in this report.
","abstract_html":"<p>This thesis describes an in vivo perfusion apparatus which can continuously deliver controlled quantities of solutions to localized, contralateral intra oral areas in albino rats. Three animals were per fused on the experimental area (the mesial sulcular area of the maxillary first molar) on one side with tap water and trypan blue. Localization of the solution delivered and minimal histological changes due to the presence of the apparatus was demonstrated on these animals. A 1% solution of trypsin was perfused on the experimental area of one animal for one day at the rate of 4.8 ml/day and on another animal for four days at the same rate. A 1% pronase solution was perfused for four days on one animal at the rate of .96 ml/day and another animal at 4.8 ml/day. The appropriate control solutions were delivered on the contralateral side of each animal undergoing enzyme perfusion. Data obtained indicated moderate inflammatory changes were noted on the side receiving the enzyme. More meaningful results should be obtained with the modifications discussed in this report.</p>","abstract_has_math":false,"creators":["Dornan, Don"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Periodontics","degree_department":null,"school":null,"contributors":["Richard C. Oliver","Edwin M. Collins","G. Conrad Hornbuckle","Eugene Rathbun","John De Vincenzo"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1969,"date_issued":"1969-05-01T07:00:00Z","date_published":"1969-05-01T07:00:00Z","updated_at":"2026-07-24T02:54:17Z","subjects":["Periodontics and Periodontology","Gingival Diseases"],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/1189","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Richard C. Oliver","Edwin M. Collins","G. Conrad Hornbuckle","Eugene Rathbun","John De Vincenzo"]},{"key":"dc:creator","label":"Author","values":["Dornan, Don"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Periodontics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Periodontics and Periodontology","Gingival Diseases"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/1189"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["This thesis describes an in vivo perfusion apparatus which can continuously deliver controlled quantities of solutions to localized, contralateral intra oral areas in albino rats. Three animals were per fused on the experimental area (the mesial sulcular area of the maxillary first molar) on one side with tap water and trypan blue. Localization of the solution delivered and minimal histological changes due to the presence of the apparatus was demonstrated on these animals. A 1% solution of trypsin was perfused on the experimental area of one animal for one day at the rate of 4.8 ml/day and on another animal for four days at the same rate. A 1% pronase solution was perfused for four days on one animal at the rate of .96 ml/day and another animal at 4.8 ml/day. The appropriate control solutions were delivered on the contralateral side of each animal undergoing enzyme perfusion. Data obtained indicated moderate inflammatory changes were noted on the side receiving the enzyme. More meaningful results should be obtained with the modifications discussed in this report.
"]},{"key":"dc:title","label":"Title","values":["An In Vivo Perfusion Apparatus for Studies on Gingival Disease : Assessment by a Perfusion of Nonspecific Proteolytic Enzymes"]}]}],"canonical_facts":{"dc:contributor":["Richard C. Oliver","Edwin M. Collins","G. Conrad Hornbuckle","Eugene Rathbun","John De Vincenzo"],"dc:creator":["Dornan, Don"],"dc:description.abstract":["This thesis describes an in vivo perfusion apparatus which can continuously deliver controlled quantities of solutions to localized, contralateral intra oral areas in albino rats. Three animals were per fused on the experimental area (the mesial sulcular area of the maxillary first molar) on one side with tap water and trypan blue. Localization of the solution delivered and minimal histological changes due to the presence of the apparatus was demonstrated on these animals. A 1% solution of trypsin was perfused on the experimental area of one animal for one day at the rate of 4.8 ml/day and on another animal for four days at the same rate. A 1% pronase solution was perfused for four days on one animal at the rate of .96 ml/day and another animal at 4.8 ml/day. The appropriate control solutions were delivered on the contralateral side of each animal undergoing enzyme perfusion. Data obtained indicated moderate inflammatory changes were noted on the side receiving the enzyme. More meaningful results should be obtained with the modifications discussed in this report.
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/1189"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Periodontics and Periodontology","Gingival Diseases"],"dc:title":["An In Vivo Perfusion Apparatus for Studies on Gingival Disease : Assessment by a Perfusion of Nonspecific Proteolytic Enzymes"],"thesis:degree_discipline":["Periodontics"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:54:17Z"}