Telomerase Activity in Prostatic Fluid and Tissue as a Marker for Prostate Cancer

<p>Telomerase is a ribonucleoprotein enzyme that is able to add telomeric repeats to the ends of eukaryotic DNA to stabilize chromosomes. Telomerase is active in germline cells, stem cells and tumor cells, but not in most human somatic cells. In this study we report the extended use of the Telomeric Repeat Amplification Protocol (TRAP) assay for the detection of telomerase activity in prostate needle biopsy specimens and freshly collected prostatic fluid. Clinical application of a newly developed PCR-ELISA telomerase assay method is also evaluated. Multiple sextant needle biopsy specimens from 56 subjects and prostatic secretion specimens from 72 subjects, with or without prostate cancer (PCa), were examined. In the needle core group the test results showed 90% sensitivity and 78% specificity for the detection of prostate cancer. In the prostatic secretion group the sensitivity was 77% and the specificity 84%. Statistical analysis of both groups revealed that higher positive rates and stronger telomerase activity were associated with higher Gleason scores and tumor anaplasia. The data from a comparison of telomerase activity with volume of cancer in the needle cores demonstrated that greater telomerase activity was always coupled with higher cancer volume. We also demonstrated a high correlation between telomerase activity in prostatic secretion specimens and serum PSA values. In a comparison study, we tested telomerase activity in 48 prostate needle cores containing or not containing cancer cells, and in 18 prostatic fluid specimens from patients with or without PCa by both the PCR-ELIS A method and the TRAP assay. The assay results showed high correlation between two methods.</p> <p>In conclusion, telomerase activity was reliably detected by each method in specimens of both types. It may be clinically meaningful to apply this new assay system for determining telomerase activity because by its sensitivity it provides valuable information for early diagnosis, and beyond that for therapeutic evaluation of prostate cancer patients.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Year

2000

Author dc:creator

• Wang, Zhilian

Contributors dc:contributor

• Richard E. Beltz
• E. Clifford Herrmann
• William H. R. Langridge
• Herbert C. Ruckle
• Charles W. Slattery

• Biochemistry
• Prostatic Neoplasms -- diagnosis; Telomerase -- antagonists and inhibitors; RNA-Directed DNA Polymerase; Prostate -- cytology

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/1164

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-2644