A Restriction Analysis of Klebsiella Pneumoniae Phage SBS
A Klebsiella phage was isolated from the San Bernardino sewage and its physiochemical properties analyzed. The present study found the density of the phage to be 1.44 g/ml, the burst size to be 10, and the phage had a latent period of 20-25 minutes. The DNA was found to be double stranded and linear. Its molecular weight was 41.66 + 0.72 kilobase pairs. The DNA was digested with a battery of endoncleases and partial restriction maps determined. The potential of the phage as a vector for Klebsiella is discussed in the text.
Microbiology, Klebsiella pneumoniae -- analysis; DNA, Recombinant; Cloning, Molecular
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