The Effects of Certain Oral Bacteria on the In Vitro Growth of Bacteroides gingivalis

<p>The effect of nine gram positive and grain negative cocci and bacilli, which are indigenous to the oral cavity, on the in vitro growth of <em>Bacteroides gingivalis</em> was examined in this study. A pure culture of <em>B. gingivalis</em> is was obtained from the American Type Culture Collection. The nine oral test organisms were <em>Streptococcus mitis</em>, <em>Streptococcus mutans,</em> <em>Streptococcus faecalis,</em> <em>Staphylococcus epidermidis,</em> <em>Staphylococcus aureus</em>, <em>Corynebacteriurn hofmanii</em>, <em>Lactobacillus acidophilus</em>, <em>Branhamella catarrhalis</em>, and <em>Escherichia coli</em>. The effect on the growth of <em>B. gingivalis</em> of the nine test organisms was evaluated on a solid medium by use of a satellite phenomenon study and in a liquid medium by use of an optical density study. Incubation was conducted under aerobic conditions for seven days at 30°C.</p> <p>The sterile filtrates of trypticase soy broth which was used as the culture medium during incubation and growth of the nine provider organisms were evaluated for the presence of menadione by use of high pressure liquid chromatography.</p> <p>The results of the satellite phenomenon study indicate that <em>B. gingivalis</em> (ATCC #33277) requires menadione (vit K) for in vitro growth. Of the nine organisms tested, the gram positive cocci were more efficient "provider" organisms than the gram positive and gram negative bacilli.</p> <p>The results of the optical density study also indicate that <em>B. gingivalis</em> (ATCC #33277) requires menadione for in vitro growth. Of the nine provider organisms tested, only <em>S. mutans</em> demonstrated a significant (p < 0.05) increase in the growth of <em>B. gingivalis</em> as compared to the positive control.</p> <p>The high pressure liquid chromatography analysis indicates that of the nine provider organisms tested, only <em>S. mutans</em> produced a substance into its growth environment which was similar in molecular structure and polarity to menadione. <em>B. gingivalis </em>is is an important pathogen in oral-facial infections. It requires menadione for growth. The pathogenicity of <em>B. gingivalis</em> appears to be the result of a synergistic mixed infection which contains facultative gram positive cocci, especially <em>S. mutans</em>, which synthesize menadione or a similar substance, thus making it available at the site of infection as a needed growth requirement.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Microbiology

Year

1986

Author dc:creator

• Morrow, Steven G.

Contributors dc:contributor

• James D. Kettering
• Benjamin H.S. Lau
• Mahmoud Torabinejad

• Bacteriology
• Laboratory and Basic Science Research
• Microbiology
• Oral Biology and Oral Pathology
• Bacteroides -- growth & development; Vitamin K

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/1664

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-2353