Infective oncospheres of Oochorlstica osheroffi and Oochoristica scelopori were compared using fifteen histochemical tests. Vital stains were also used.
Carnoy’s-fixed gravid proglottids were vacuum-embedded in a paraffin-piccolyte mixture and cut transversely at 6 microns. Sections were tested for the presence of phosphatases, proteins, sclerotin precursors, nucleic acids, carbohydrates and acid mucopolysaccharides.
Oncospheres were histochemlcally very similar. Heavy concentrations of mucoproteins and glycoproteins were detected in the oncospheral coat of O. scelopori. Acid phosphatase was found in the embryophore of O. osheroffi, but could not be demonstrated in O. scelopori. No evidence of a sclerotized egg capsule was found in either species.
In both species studied, the embryophore is formed by the degeneration of cells of the inner envelope. The outer envelope is formed by two macromeres and gradually accumulates many large lipid droplets. The possible origin of these droplets is discussed.
This study confirms the often hypothesized cellular origin of the embryophore, outer envelope and Inner envelope. These structures were previously reported as being formed acellularly.
","abstract_html":"<p>Infective oncospheres of <em>Oochorlstica osheroffi</em> and <em>Oochoristica scelopori</em> were compared using fifteen histochemical tests. Vital stains were also used.</p> <p>Carnoy’s-fixed gravid proglottids were vacuum-embedded in a paraffin-piccolyte mixture and cut transversely at 6 microns. Sections were tested for the presence of phosphatases, proteins, sclerotin precursors, nucleic acids, carbohydrates and acid mucopolysaccharides.</p> <p>Oncospheres were histochemlcally very similar. Heavy concentrations of mucoproteins and glycoproteins were detected in the oncospheral coat of <em>O. scelopori</em>. Acid phosphatase was found in the embryophore of <em>O. osheroffi</em>, but could not be demonstrated in <em>O. scelopori</em>. No evidence of a sclerotized egg capsule was found in either species.</p> <p>In both species studied, the embryophore is formed by the degeneration of cells of the inner envelope. The outer envelope is formed by two macromeres and gradually accumulates many large lipid droplets. The possible origin of these droplets is discussed.</p> <p>This study confirms the often hypothesized cellular origin of the embryophore, outer envelope and Inner envelope. These structures were previously reported as being formed acellularly.</p>","abstract_has_math":false,"creators":["Coulter, Gary R."],"institution":null,"degree_name":"Master of Arts (MA)","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Elmer A. Widmer","Edward D. Wagner","Berney H. Neufeld"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1972,"date_issued":"1972-06-01T07:00:00Z","date_published":"1972-06-01T07:00:00Z","updated_at":"2026-07-24T02:54:01Z","subjects":["Biochemistry, Biophysics, and Structural Biology","Biology","Laboratory and Basic Science Research","Parasitology","Cestoda -- classification"],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/1522","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Elmer A. Widmer","Edward D. Wagner","Berney H. Neufeld"]},{"key":"dc:creator","label":"Author","values":["Coulter, Gary R."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Arts (MA)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biochemistry, Biophysics, and Structural Biology","Biology","Laboratory and Basic Science Research","Parasitology","Cestoda -- classification"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/1522"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Infective oncospheres of Oochorlstica osheroffi and Oochoristica scelopori were compared using fifteen histochemical tests. Vital stains were also used.
Carnoy’s-fixed gravid proglottids were vacuum-embedded in a paraffin-piccolyte mixture and cut transversely at 6 microns. Sections were tested for the presence of phosphatases, proteins, sclerotin precursors, nucleic acids, carbohydrates and acid mucopolysaccharides.
Oncospheres were histochemlcally very similar. Heavy concentrations of mucoproteins and glycoproteins were detected in the oncospheral coat of O. scelopori. Acid phosphatase was found in the embryophore of O. osheroffi, but could not be demonstrated in O. scelopori. No evidence of a sclerotized egg capsule was found in either species.
In both species studied, the embryophore is formed by the degeneration of cells of the inner envelope. The outer envelope is formed by two macromeres and gradually accumulates many large lipid droplets. The possible origin of these droplets is discussed.
This study confirms the often hypothesized cellular origin of the embryophore, outer envelope and Inner envelope. These structures were previously reported as being formed acellularly.
"]},{"key":"dc:title","label":"Title","values":["The Comparative Histochemistry of the Infective Oncospheres of OOCHORISTICA OSHEROFFI Meggitt, 1934, and OOCHORISTICA SCELOPORI Voge and Fox, 1950, (Cyclophyllidea: Linstowiidae) with Notes on the Origins of the Egg Membranes"]}]}],"canonical_facts":{"dc:contributor":["Elmer A. Widmer","Edward D. Wagner","Berney H. Neufeld"],"dc:creator":["Coulter, Gary R."],"dc:description.abstract":["Infective oncospheres of Oochorlstica osheroffi and Oochoristica scelopori were compared using fifteen histochemical tests. Vital stains were also used.
Carnoy’s-fixed gravid proglottids were vacuum-embedded in a paraffin-piccolyte mixture and cut transversely at 6 microns. Sections were tested for the presence of phosphatases, proteins, sclerotin precursors, nucleic acids, carbohydrates and acid mucopolysaccharides.
Oncospheres were histochemlcally very similar. Heavy concentrations of mucoproteins and glycoproteins were detected in the oncospheral coat of O. scelopori. Acid phosphatase was found in the embryophore of O. osheroffi, but could not be demonstrated in O. scelopori. No evidence of a sclerotized egg capsule was found in either species.
In both species studied, the embryophore is formed by the degeneration of cells of the inner envelope. The outer envelope is formed by two macromeres and gradually accumulates many large lipid droplets. The possible origin of these droplets is discussed.
This study confirms the often hypothesized cellular origin of the embryophore, outer envelope and Inner envelope. These structures were previously reported as being formed acellularly.
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/1522"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Biochemistry, Biophysics, and Structural Biology","Biology","Laboratory and Basic Science Research","Parasitology","Cestoda -- classification"],"dc:title":["The Comparative Histochemistry of the Infective Oncospheres of OOCHORISTICA OSHEROFFI Meggitt, 1934, and OOCHORISTICA SCELOPORI Voge and Fox, 1950, (Cyclophyllidea: Linstowiidae) with Notes on the Origins of the Egg Membranes"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Arts (MA)"]},"updated_at":"2026-07-24T02:54:01Z"}