Effects of TRYPANOSOMA MUSCULI Kendall, 1906 Immune Serum on the Reproduction of TRICHINELLA SPIRALIS (Owen, 1835) Railliet, 1896 in Mice
type: CreativeWork
description: The possible effects of ablastin, a reproduction-inhibiting antibody which is produced during the infection of the blood parasite, Trypanosoma musculi in the mouse, were investigated by fractionating the parasite into a supernatant fraction (designated as Group A test mice) and a pellet fraction (Group B test mice). These groups A and B mice were injected with the respective fractions, plus Freund’s Complete Adjuvant (FCA). Control mice were injected with FCA only. Sera from these three groups of mice were removed after the Group A and Group B mice were shown to be negative to T. musculi challenge, and Group A and Group B mice were given their passive immune serum injections. The FCA control mice were similiarily given the FCA serum injections. These FCA control mice were given the same dose inoculum of Trichinella spiralis larvae by gastric gavage. On day 26 post T. spiralis gavaging, all of the mice were sacrificed, and the nematode larvae digested from the muscles of each mouse. The mean number of larvae recovered from the FCA control mice was significantly higher (46,050) than the means of both Group A (12,427) and Group B (11,144) , thus suggesting that the immune serum containing ablastin transferred to the test mice, suppressed the development of larvae as compared to the FCA control mice. A group of control mice which received normal mouse serum only, and given larvae by gavage at the same dosage level and at the same time, showed a markedly lower mean number of larvae (5,669) as compared to the FCA control mice, and about half as many The mechanism in operation which permits the FCA to greatly enhance the larval development in the host, possibly by suppressing the expulsion of the adult worms from the larvae as the test mice. Groups A and B. host's intestine, is not known. However, it must be considered as playing a major role, and suggests an area of future study. When the mean larval count of the control mice, which received only normal mouse serum, is co…
abstract: The possible effects of ablastin, a reproduction-inhibiting antibody which is produced during the infection of the blood parasite, Trypanosoma musculi in the mouse, were investigated by fractionating the parasite into a supernatant fraction (designated as Group A test mice) and a pellet fraction (Group B test mice). These groups A and B mice were injected with the respective fractions, plus Freund’s Complete Adjuvant (FCA). Control mice were injected with FCA only. Sera from these three groups of mice were removed after the Group A and Group B mice were shown to be negative to T. musculi challenge, and Group A and Group B mice were given their passive immune serum injections. The FCA control mice were similiarily given the FCA serum injections. These FCA control mice were given the same dose inoculum of Trichinella spiralis larvae by gastric gavage. On day 26 post T. spiralis gavaging, all of the mice were sacrificed, and the nematode larvae digested from the muscles of each mouse. The mean number of larvae recovered from the FCA control mice was significantly higher (46,050) than the means of both Group A (12,427) and Group B (11,144) , thus suggesting that the immune serum containing ablastin transferred to the test mice, suppressed the development of larvae as compared to the FCA control mice. A group of control mice which received normal mouse serum only, and given larvae by gavage at the same dosage level and at the same time, showed a markedly lower mean number of larvae (5,669) as compared to the FCA control mice, and about half as many The mechanism in operation which permits the FCA to greatly enhance the larval development in the host, possibly by suppressing the expulsion of the adult worms from the larvae as the test mice. Groups A and B. host's intestine, is not known. However, it must be considered as playing a major role, and suggests an area of future study. When the mean larval count of the control mice, which received only normal mouse serum, is co…
author: Chafin, Samuel L.
language: English
keywords: Animal Experimentation and Research, Laboratory and Basic Science Research, Microbiology, Parasitic Diseases, Parasitology, Trypanosoma; Trichinella
date published: 1978-06-01T07:00:00Z
date modified: 2026-07-24T02:53:44Z
provider: Loma Linda University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Loma Linda University