Carbon Dioxide Fixation in Schistosoma Mansoni

<p>Adults of the blood fluke <em>Schistosoma mansoni</em> were perfused from the hepatic portal system of CF1 female mice sixty to seventy days post infection. The worms were then incubated in Eagle’s minimal essential medium with glutamate and 0.03 mg/100 ml of nonlabelled aspartic acid. To this medium was added 10 μc of either NaH¹⁴0₃, glucose-UC¹⁴ or aspartate-4-C¹⁴. The worms were found to fix carbon from the NaH¹⁴0₃ and to incorporate the glucose and aspartate. The amount of activity recovered in five different chemical fractions after two hours incubation was determined for each radiochemical used and compared to determine the relative significance of carbon dioxide in <em>Schistosoma mansoni</em> metabolism.</p> <p>All five fractions which were separated from worms incubated in glucose were significantly different from the control samples. The worms incubated in aspartate and sodium bicarbonate incorporated these compounds into every fraction except the ethanol fraction. Worms incubated in both aspartate and sodium bicarbonate had the greatest amount of incorporation of label in the protein fraction with the least amount in the ethanol soluble fraction.</p> <p>Under the experimental conditions employed glucose appears to be the most important source of carbon when compared with either aspartate or bicarbonate.</p>

Name thesis:degree_name

Master of Arts (MA)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year

1967

Author dc:creator

• Carter, Clint Earl

Contributors dc:contributor

• Ariel A. Roth
• Edward D. Wagner
• R. Bruce Wilcox

• Animal Experimentation and Research
• Biology
• Laboratory and Basic Science Research
• Parasitic Diseases
• Parasitology
• Carbon Dioxide; Schistosoma mansoni

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/1451

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-2223