Concurrent Infection of TRYPANOSOMA MUSCULI Kendell, 1906, and SCHISTOSOMA MANSONI Sambon, 1907 in Mice

<p>The possible effects of ablastin on <em>Schistosoma mansoni</em> were investigated by (1) establishing a concurrent infection of <em>Trypanosoma musculi</em> and <em>Schistosoma mansoni</em> in mice, and (2) by obtaining <em>Trypanosoma lewisi</em> immune rat serum (ablastin) and injecting it into <em>S. mansoni</em> infected mice. These procedures were followed on days 4, 14 and 28 of <em>S. mansoni</em> infection. For the groups that were given immune rat serum, controls were kept into which normal rat serum was injected.</p> <p>Ablastin does not have any effect on the development or morphology of the <em>S. mansoni</em> worms as tested in this study. Although ablastin inhibits glycolytic pathway enzymes in young trypanosomes, it does not seem to affect those of the blood fluke. Effects of schistosome membrane function must also be ruled out as no reduction in worm burden and no morphological abnormalities were observed. Both immune and normal rat serum caused an increase in the number of worms recovered. This is most probably due to a serum sickness type reaction in the mice, caused by the foreign rat protein. This is supported by preliminary experiments which showed that mice given immune rat serum every third day died of anaphylactic shock on the twelfth day, a few minutes after the fifth injection was given.</p> <p>Starting both <em>T. musculi</em> and <em>S. mansoni</em> infections at the same time as well as establishing a high titer of ablastin by introducing the <em>T. musculi</em> infection 8 to 10 days prior to the schistosome infection. would establish the effect of ablastin on the penetration of <em>S. mansoni</em> cercariae.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Microbiology

Year

1976

Author dc:creator

• Andeberhan, Berhane

Contributors dc:contributor

• Edward D. Wagner
• Raymond E. Ryckman
• Clifford E. Herrmann

• Animal Experimentation and Research
• Microbiology
• Parasitology
• Microbiology; Schistosoma mansoni; Trypanosoma

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/1442

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-2214