Mother’s milk is recommended by the nutritional and pediatric communities as the best food for suckling infants. For non breast-fed infants, bovine milk-based formulas have been regarded as a suitable substitute. In the early 1900's, physicians found that foreign proteins in cow milk were responsible for gastrointestinal disease and even digestive collapse of the newborn fed a cow milk-based diet16. The allergenicity of cow milk for the human infant appears to be caused predominantly by p-casein17. In this research, the cDNA encoding human p-casein was introduced into potato cells under the control of the bidirectional, auxin-inducible mannopine synthase gene (mas) promoter by Agrobacterium turnefaciens-mediated leaf disc transformation methods. The presence of the p-casein gene and its transcript in regenerated transgenic plants were confirmed by PCR and RT-PCR analysis respectively. Human p-casein protein was detected in leaves of transgenic plants by immunoblot analysis. The p-casein protein produced by the plants migrated as a single band with a molecular mass of about 30 KDa and was approximately 0.01% of the total soluble protein in transgenic potato tissue.
","abstract_html":"<p>Mother’s milk is recommended by the nutritional and pediatric communities as the best food for suckling infants. For non breast-fed infants, bovine milk-based formulas have been regarded as a suitable substitute. In the early 1900's, physicians found that foreign proteins in cow milk were responsible for gastrointestinal disease and even digestive collapse of the newborn fed a cow milk-based diet<sup>16</sup>. The allergenicity of cow milk for the human infant appears to be caused predominantly by p-casein<sup>17</sup>. In this research, the cDNA encoding human p-casein was introduced into potato cells under the control of the bidirectional, auxin-inducible mannopine synthase gene (mas) promoter by <em>Agrobacterium turnefaciens</em>-mediated leaf disc transformation methods. The presence of the p-casein gene and its transcript in regenerated transgenic plants were confirmed by PCR and RT-PCR analysis respectively. Human p-casein protein was detected in leaves of transgenic plants by immunoblot analysis. The p-casein protein produced by the plants migrated as a single band with a molecular mass of about 30 KDa and was approximately 0.01% of the total soluble protein in transgenic potato tissue.</p>","abstract_has_math":false,"creators":["Roberts, Wendy"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Dissertation","degree_discipline":"Microbiology, Molecular Biology and Biochemistry","degree_department":null,"school":null,"contributors":["William H. R. Langrides","Allan P. Escher","Anthony J. Zuccarelli"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1996,"date_issued":"1996-06-01T07:00:00Z","date_published":"1996-06-01T07:00:00Z","updated_at":"2026-07-24T02:53:20Z","subjects":["Microbiology","Molecular Genetics","Milk Proteins; Milk, Human; Caseins; Plants, Transgenic; Plant Proteins"],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/762","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["William H. R. Langrides","Allan P. Escher","Anthony J. 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The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/762"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Mother’s milk is recommended by the nutritional and pediatric communities as the best food for suckling infants. For non breast-fed infants, bovine milk-based formulas have been regarded as a suitable substitute. In the early 1900's, physicians found that foreign proteins in cow milk were responsible for gastrointestinal disease and even digestive collapse of the newborn fed a cow milk-based diet16. The allergenicity of cow milk for the human infant appears to be caused predominantly by p-casein17. In this research, the cDNA encoding human p-casein was introduced into potato cells under the control of the bidirectional, auxin-inducible mannopine synthase gene (mas) promoter by Agrobacterium turnefaciens-mediated leaf disc transformation methods. The presence of the p-casein gene and its transcript in regenerated transgenic plants were confirmed by PCR and RT-PCR analysis respectively. Human p-casein protein was detected in leaves of transgenic plants by immunoblot analysis. The p-casein protein produced by the plants migrated as a single band with a molecular mass of about 30 KDa and was approximately 0.01% of the total soluble protein in transgenic potato tissue.
"]},{"key":"dc:title","label":"Title","values":["Expression of the Human Milk Protein, Beta-Casein : in Transgenic Potato Plants"]}]}],"canonical_facts":{"dc:contributor":["William H. R. Langrides","Allan P. Escher","Anthony J. Zuccarelli"],"dc:creator":["Roberts, Wendy"],"dc:description.abstract":["Mother’s milk is recommended by the nutritional and pediatric communities as the best food for suckling infants. For non breast-fed infants, bovine milk-based formulas have been regarded as a suitable substitute. In the early 1900's, physicians found that foreign proteins in cow milk were responsible for gastrointestinal disease and even digestive collapse of the newborn fed a cow milk-based diet16. The allergenicity of cow milk for the human infant appears to be caused predominantly by p-casein17. In this research, the cDNA encoding human p-casein was introduced into potato cells under the control of the bidirectional, auxin-inducible mannopine synthase gene (mas) promoter by Agrobacterium turnefaciens-mediated leaf disc transformation methods. The presence of the p-casein gene and its transcript in regenerated transgenic plants were confirmed by PCR and RT-PCR analysis respectively. Human p-casein protein was detected in leaves of transgenic plants by immunoblot analysis. The p-casein protein produced by the plants migrated as a single band with a molecular mass of about 30 KDa and was approximately 0.01% of the total soluble protein in transgenic potato tissue.
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/762"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Microbiology","Molecular Genetics","Milk Proteins; Milk, Human; Caseins; Plants, Transgenic; Plant Proteins"],"dc:title":["Expression of the Human Milk Protein, Beta-Casein : in Transgenic Potato Plants"],"thesis:degree_discipline":["Microbiology, Molecular Biology and Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:53:20Z"}