The work on the active substance of Eremocarpus setigerus Benth has been previously performed by Naito and Noller. However, the investigation was not complete. Therefore we isolated and tested this substance by steam distillation and thin layer chromatography technique. A yellow sticky toxic substance was isolated with Rf values of 0.15 in benzene system and 0.57 in benzene-absolute ethanol (95:5 v/v) system.
The toxic substance which is free of halogen, nitrogen, and sulfur, is soluble in ethyl ether, ethanol, and concentrated sulfuric acid. It has a maximum absorption in the ultraviolet region that indicates the presence of unsaturation. Its infrared spectrum indicates the presence of a·saturated tertiary or a highly symmetrical secondary hydroxyl group and carbonyl group. The functional group tests also indicate the presence of ketone, ester, enol and isolated double bond groups. We believe that the enol form is attributed to the conjugation of the ketone and the ester groups at a β-carbon position.
Furthermore, the toxic substance gave a positive terpene-terpenoid test. This leads us to believe that the toxic substance might belong to the triterpenoid series.
","abstract_html":"<p>The work on the active substance of Eremocarpus setigerus Benth has been previously performed by Naito and Noller. However, the investigation was not complete. Therefore we isolated and tested this substance by steam distillation and thin layer chromatography technique. A yellow sticky toxic substance was isolated with Rf values of 0.15 in benzene system and 0.57 in benzene-absolute ethanol (95:5 v/v) system.</p> <p>The toxic substance which is free of halogen, nitrogen, and sulfur, is soluble in ethyl ether, ethanol, and concentrated sulfuric acid. It has a maximum absorption in the ultraviolet region that indicates the presence of unsaturation. Its infrared spectrum indicates the presence of a·saturated tertiary or a highly symmetrical secondary hydroxyl group and carbonyl group. The functional group tests also indicate the presence of ketone, ester, enol and isolated double bond groups. We believe that the enol form is attributed to the conjugation of the ketone and the ester groups at a β-carbon position.</p> <p>Furthermore, the toxic substance gave a positive terpene-terpenoid test. This leads us to believe that the toxic substance might belong to the triterpenoid series.</p>","abstract_has_math":false,"creators":["Lee, Shuliang"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Laurence W. Botimer","Earl W. Lathrop","William D. Leech","Maurice E. Mathisen","Wayne E. Zaugg"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1968,"date_issued":"1968-05-01T07:00:00Z","date_published":"1968-05-01T07:00:00Z","updated_at":"2026-07-24T02:53:20Z","subjects":["Biochemistry","Chemistry","Plant Sciences","Poisonous plants; Chromatographic analysis"],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/995","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Laurence W. Botimer","Earl W. Lathrop","William D. Leech","Maurice E. Mathisen","Wayne E. Zaugg"]},{"key":"dc:creator","label":"Author","values":["Lee, Shuliang"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biochemistry","Chemistry","Plant Sciences","Poisonous plants; Chromatographic analysis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/995"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The work on the active substance of Eremocarpus setigerus Benth has been previously performed by Naito and Noller. However, the investigation was not complete. Therefore we isolated and tested this substance by steam distillation and thin layer chromatography technique. A yellow sticky toxic substance was isolated with Rf values of 0.15 in benzene system and 0.57 in benzene-absolute ethanol (95:5 v/v) system.
The toxic substance which is free of halogen, nitrogen, and sulfur, is soluble in ethyl ether, ethanol, and concentrated sulfuric acid. It has a maximum absorption in the ultraviolet region that indicates the presence of unsaturation. Its infrared spectrum indicates the presence of a·saturated tertiary or a highly symmetrical secondary hydroxyl group and carbonyl group. The functional group tests also indicate the presence of ketone, ester, enol and isolated double bond groups. We believe that the enol form is attributed to the conjugation of the ketone and the ester groups at a β-carbon position.
Furthermore, the toxic substance gave a positive terpene-terpenoid test. This leads us to believe that the toxic substance might belong to the triterpenoid series.
"]},{"key":"dc:title","label":"Title","values":["Isolation and Partial Characterization of the Toxic Substance from EREMOCARPUS SETIGERUS BENTH"]}]}],"canonical_facts":{"dc:contributor":["Laurence W. Botimer","Earl W. Lathrop","William D. Leech","Maurice E. Mathisen","Wayne E. Zaugg"],"dc:creator":["Lee, Shuliang"],"dc:description.abstract":["The work on the active substance of Eremocarpus setigerus Benth has been previously performed by Naito and Noller. However, the investigation was not complete. Therefore we isolated and tested this substance by steam distillation and thin layer chromatography technique. A yellow sticky toxic substance was isolated with Rf values of 0.15 in benzene system and 0.57 in benzene-absolute ethanol (95:5 v/v) system.
The toxic substance which is free of halogen, nitrogen, and sulfur, is soluble in ethyl ether, ethanol, and concentrated sulfuric acid. It has a maximum absorption in the ultraviolet region that indicates the presence of unsaturation. Its infrared spectrum indicates the presence of a·saturated tertiary or a highly symmetrical secondary hydroxyl group and carbonyl group. The functional group tests also indicate the presence of ketone, ester, enol and isolated double bond groups. We believe that the enol form is attributed to the conjugation of the ketone and the ester groups at a β-carbon position.
Furthermore, the toxic substance gave a positive terpene-terpenoid test. This leads us to believe that the toxic substance might belong to the triterpenoid series.
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/995"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Biochemistry","Chemistry","Plant Sciences","Poisonous plants; Chromatographic analysis"],"dc:title":["Isolation and Partial Characterization of the Toxic Substance from EREMOCARPUS SETIGERUS BENTH"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:53:20Z"}