Time Delayed Protein Complementation

<p>The time delayed complementation of plant proteins was tested in weanling Sprague-Dawley male rats by measuring growth rates as well as plasma and tissue free amino acids. Diets of pinto beans with rice, corn or wheat were pair-fed in combination or alternating 4 times a day at 6 and 11 am, and 4 and 9 pm for 4 weeks. Plasma, liver and muscle samples were obtained 2 hours postprandial, a time determined most suitable for sacrifice, and analyzed for free amino acids via ion-exchange chromatography. There were no significant differences in growth of rats fed combination or alternating diets.during week 3 and 4 with the exception of the limiting corn-bean combination and alternating diets in week four. Step-wise multiple regression of the data show plasma free amino acids most correlated to growth included asparagine, 15.1% contribution; and arginine, tyrosine, histidine, 1- methylhistidine and cystathionine, each contributing <5%. The following amino acids were negatively correlated with growth: threonine, 29.2%; and leucine, phosphoethanolamine, taurine and serine <6.5%. This study confirms previous work in this laboratory which suggested that vegetable proteins fed 5 hours apart in combined or alternating fashion had equal growth promoting capacity. Contrary to expectations, the limiting amino acids in these diets -- methionine, lysine and tryptophan -- were not the postprandial plasma amino acids most related to growth rate. More study is necessary to correlate the limiting amino acids in proteins and tissue free amino acids with the growth rate of the rat. </p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Nutrition

Year

1987

Author dc:creator

• Lakusta, Diana L.

Contributors dc:contributor

• Albert Sanchez
• Richard Hubbard
• Ulma D. Register
• Gerald Shavlik

• Biochemistry
• Biostatistics
• Dietetics and Clinical Nutrition
• Complement; Vegetable Proteins; Time.

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/998

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-1841