Identification and Characterization of Aer, an Energy Sensor in Escherichia Coli

<p>This study investigated the mechanism of signal transduction by Aer, the aerotaxis transducer in <em>E.coli</em>. A computerized search of GenBank revealed an open reading frame at 69.1 min on the E.co//chromosome that had a C-terminal fragment with 96.7% identity to the highly conserved domain of Tsr. The open reading frame was renamed aer for aerotaxis and energy responses. When the aer gene was inactivated, approximately 50% of aerotaxis responses were eliminated. When both <em>aer</em> and <em>fsr</em> were inactivated, aerotaxis, redox taxis and glycerol taxis were completely abolished. Expressing Aer in aer fsr cells restored aerotaxis responses and the duration of responses progressively lengthened with the amount of Aer overexpression. These results confirmed that Aer and Tsr are independently sense intracellular energy levels and transduce oxygen, redox and energy signals.</p> <p>Sequence analysis of Aer revealed a PAS domain in the N-terminus of the protein. PAS domains are input modules found in a large family of light, oxygen, voltage and redox sensors. Forty two conserved and non-conserved Aer PAS domain residues were serially replaced with cysteine to measure their effect on sensing and signaling by Aer. This study identified Aer PAS domain residues important for FAD binding, sensing, signal bias and signal output. A 3D model of the Aer PAS domain was generated using PYP as the structural template. Important residues were mapped on the Aer PAS model and an active site for FAD binding and signal transduction was proposed.</p> <p>Different approaches were used to identify the upstream component in the electron transport system that signals to Aer. The yeast two hybrid system was utilized to assay interactions between Aer and all other proteins encoded by a <em>E.coli</em> genomic library. The role of NADH dehydrogenase I in aerotaxis was also investigated by measuring responses of <em>nuo fsr</em> cells. The study indicated that Aer can function in the absence of NADH dehydrogenase I.</p> <p>We sought to isolate and purify the soluble N-terminus of Aer. However, the N-terminus peptide formed inclusion bodies. Co-expressing the GroESL chaperonins alleviated inclusion body formation and increased the solubility of Aer.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Year

2000

Author dc:creator

• Rebbapragada, Anuradha

Contributors dc:contributor

• Barry L. Taylor
• Hansel Fletcher
• John J. Rossi
• Igor B. Zhulin
• Anthony J. Zuccarelli

• Microbiology
• Molecular Genetics
• Penicillinase; Signal Transduction; Gene Expression Regulation, Bacterial; Escherichia Coli.

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/693

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-1817