Cocaine Induces Apoptosis in Coronary Artery Endothelial Cells: Cellular and Molecular Mechanisms

<p>Cocaine abuse has been associated with numerous cardiovascular consequences, such as depressed myocardial contractility, elevated blood pressure, coronary artery vasoconstriction, and myocardial ischemia and infarction. The multifactorial effects of cocaine often contribute to its sympathomimetic function. However, the direct cytotoxic effects of cocaine on the cardiovascular system, especially vascular endothelium, and the cellular and molecular mechanisms remain unknown. The present study was designed to examine the general hypothesis that cocaine induces apoptosis of coronary artery endothelium by suppressing nitric oxide synthesis, stimulating cytochrome c release from mitochondria and subsequently activation of the caspases cascade, which lead to endothelial cell apoptosis. We found that cocaine induces apoptosis of endothelial cells in a dose- and time-dependent manner. The induction of apoptosis by coaine is through the activation of mitochondria-mediated apoptotic pathway. Cocaine-induced mitochondrial cytochrome c release is via bax translocation and decrease of bel-2 level. The decrease of NO production in endothelial cells exposed to cocaine may amplify the apoptotic signals. The studies not only provide a mechanistic basis of endothelium damage in response to cocaine but also enhance our basic understanding of endothelial apoptosis in general and improve our understanding of coronary ischemia, coronary atherosclerosis, and myocardial ischemia and infarction from a new prospective.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Pharmacology

Year

2001

Author dc:creator

• He, Jiale

Contributors dc:contributor

• Lubo Zhang
• John N. Buchholz
• Carlos A. Casiano
• Charles A. Ducsay
• Raymond D. Gilbert

• Medical Pharmacology
• Cocaine -- pharmacology; Endothelium, Vascular -- cytology; Vastrictor Agentsocons -- pharmacology; Apoptosis -- drug effects -- physiology; Coronary Vessels -- cytology -- drug effects -- metabolism.

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/574

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-1746