Insulin-like growth factor binding protein-5 (IGFBP-5) is produced by osteoblasts and potentiates IGF mitogenic actions in osteoblast cultures. Progesterone (PG) increases osteoblast proliferation and increases osteoblast IGFBP-5 expression. Mechanisms underlying PG induction of IGFI3P-5 expression were investigated in U2 human osteosarcoma cells. In nuclear run-on analyses, PG increased IGF)3P-5 gene transcription to 400% of control. To define the underlying mechanisms, the human IGF13P-5 gene proximal promoter was cloned and sequenced. The proximal 500 bp of this region contains TATA and CAAT boxes, five putative PG response element (PRE) half-sites and two tandem CACCC boxes. Chloramphenicol acetyltransferase (CAT) reporter constructs containing up to 753 bp of the human IGFBP-5 gene 5'-flanking sequence were made. In U2 cells transfected with reporter construct pCAT753 containing positions -753 to t23, PG increased CAT activity to 134% of untreated control activity (P
","abstract_html":"<p>Insulin-like growth factor binding protein-5 (IGFBP-5) is produced by osteoblasts and potentiates IGF mitogenic actions in osteoblast cultures. Progesterone (PG) increases osteoblast proliferation and increases osteoblast IGFBP-5 expression. Mechanisms underlying PG induction of IGFI3P-5 expression were investigated in U2 human osteosarcoma cells. In nuclear run-on analyses, PG increased IGF)3P-5 gene transcription to 400% of control. To define the underlying mechanisms, the human IGF13P-5 gene proximal promoter was cloned and sequenced. The proximal 500 bp of this region contains TATA and CAAT boxes, five putative PG response element (PRE) half-sites and two tandem CACCC boxes. Chloramphenicol acetyltransferase (CAT) reporter constructs containing up to 753 bp of the human IGFBP-5 gene 5'-flanking sequence were made. In U2 cells transfected with reporter construct pCAT753 containing positions -753 to t23, PG increased CAT activity to 134% of untreated control activity (P</p>","abstract_has_math":false,"creators":["Boonyaratanakornicit, Viroj"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Thomas A. Linkhart","Donna D. Strong","Subburaman Mohan","R. Bruce Wilcox","Aladar A. Szalay","John F. Sands"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1996,"date_issued":"1996-06-01T07:00:00Z","date_published":"1996-06-01T07:00:00Z","updated_at":"2026-07-24T02:52:59Z","subjects":["Biochemistry","Biochemistry, Biophysics, and Structural Biology","Progesterone; Insulin-Like Growth-Factor-Binding-Protein 5; Reverse Transcriptions; Osteoblasts"],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/658","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Thomas A. Linkhart","Donna D. Strong","Subburaman Mohan","R. Bruce Wilcox","Aladar A. Szalay","John F. Sands"]},{"key":"dc:creator","label":"Author","values":["Boonyaratanakornicit, Viroj"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biochemistry","Biochemistry, Biophysics, and Structural Biology","Progesterone; Insulin-Like Growth-Factor-Binding-Protein 5; Reverse Transcriptions; Osteoblasts"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/658"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Insulin-like growth factor binding protein-5 (IGFBP-5) is produced by osteoblasts and potentiates IGF mitogenic actions in osteoblast cultures. Progesterone (PG) increases osteoblast proliferation and increases osteoblast IGFBP-5 expression. Mechanisms underlying PG induction of IGFI3P-5 expression were investigated in U2 human osteosarcoma cells. In nuclear run-on analyses, PG increased IGF)3P-5 gene transcription to 400% of control. To define the underlying mechanisms, the human IGF13P-5 gene proximal promoter was cloned and sequenced. The proximal 500 bp of this region contains TATA and CAAT boxes, five putative PG response element (PRE) half-sites and two tandem CACCC boxes. Chloramphenicol acetyltransferase (CAT) reporter constructs containing up to 753 bp of the human IGFBP-5 gene 5'-flanking sequence were made. In U2 cells transfected with reporter construct pCAT753 containing positions -753 to t23, PG increased CAT activity to 134% of untreated control activity (P
"]},{"key":"dc:title","label":"Title","values":["Progesterone Regulation of Insulin like-Growth Factor : Binding Protein-5 Gene Transcription in Human Osteoblasts"]}]}],"canonical_facts":{"dc:contributor":["Thomas A. Linkhart","Donna D. Strong","Subburaman Mohan","R. Bruce Wilcox","Aladar A. Szalay","John F. Sands"],"dc:creator":["Boonyaratanakornicit, Viroj"],"dc:description.abstract":["Insulin-like growth factor binding protein-5 (IGFBP-5) is produced by osteoblasts and potentiates IGF mitogenic actions in osteoblast cultures. Progesterone (PG) increases osteoblast proliferation and increases osteoblast IGFBP-5 expression. Mechanisms underlying PG induction of IGFI3P-5 expression were investigated in U2 human osteosarcoma cells. In nuclear run-on analyses, PG increased IGF)3P-5 gene transcription to 400% of control. To define the underlying mechanisms, the human IGF13P-5 gene proximal promoter was cloned and sequenced. The proximal 500 bp of this region contains TATA and CAAT boxes, five putative PG response element (PRE) half-sites and two tandem CACCC boxes. Chloramphenicol acetyltransferase (CAT) reporter constructs containing up to 753 bp of the human IGFBP-5 gene 5'-flanking sequence were made. In U2 cells transfected with reporter construct pCAT753 containing positions -753 to t23, PG increased CAT activity to 134% of untreated control activity (P
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/658"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Biochemistry","Biochemistry, Biophysics, and Structural Biology","Progesterone; Insulin-Like Growth-Factor-Binding-Protein 5; Reverse Transcriptions; Osteoblasts"],"dc:title":["Progesterone Regulation of Insulin like-Growth Factor : Binding Protein-5 Gene Transcription in Human Osteoblasts"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T02:52:59Z"}