We examined the major possible mechanisms for the left shift of the BK channel I-V relationship in native basilar artery myocytes from the two LTH groups. These mechanisms included: differential expression of the accessary BK -1 subunit; differential phosphorylation of the BK subunit; and splice variation of the BK subunit. Using molecular cloning, heterologous expression, and patch-clamp electrophysiology techniques, we elucidated a mechanism that, at least in part, contributes to the differences we observed between channels from native normoxic and LTH myocytes.
","abstract_html":"<p>We examined the major possible mechanisms for the left shift of the BK channel I-V relationship in native basilar artery myocytes from the two LTH groups. These mechanisms included: differential expression of the accessary BK -1 subunit; differential phosphorylation of the BK subunit; and splice variation of the BK subunit. Using molecular cloning, heterologous expression, and patch-clamp electrophysiology techniques, we elucidated a mechanism that, at least in part, contributes to the differences we observed between channels from native normoxic and LTH myocytes.</p>","abstract_has_math":false,"creators":["Tao, Xiaoxiao"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation","degree_discipline":"Basic Sciences","degree_department":null,"school":null,"contributors":["Hessinger, David A.","Buchholz, John N.","Longo, Lawrence D.","Mohan, Subburaman","Watts, Kylie J.","Zhang, Lubo"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-06-01T07:00:00Z","date_published":"2015-06-01T07:00:00Z","updated_at":"2026-07-24T02:52:27Z","subjects":["Chemical and Pharmacologic Phenomena","Medical Pharmacology","Medicine and Health Sciences","Anoxia; Hypoxia-Ischemia - Brain; Acclimatization; Altitude Sickness; Adaptation - Physiological; Myocytes; Potassium Channels - Calcium-Activated; Large-Conductance Calcium-activated Potassium Channels; Calcium Channel Blockers; Ion Channel Gating;","Left Shift of the BK Channel; Basilar Artery Myocytes; Molecular Cloning; Heterologous Express; Patch-Clamp Electrophysiology;"],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/269","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hessinger, David A.","Buchholz, John N.","Longo, Lawrence D.","Mohan, Subburaman","Watts, Kylie J.","Zhang, Lubo"]},{"key":"dc:creator","label":"Author","values":["Tao, Xiaoxiao"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Basic Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemical and Pharmacologic Phenomena","Medical Pharmacology","Medicine and Health Sciences","Anoxia; Hypoxia-Ischemia - Brain; Acclimatization; Altitude Sickness; Adaptation - Physiological; Myocytes; Potassium Channels - Calcium-Activated; Large-Conductance Calcium-activated Potassium Channels; Calcium Channel Blockers; Ion Channel Gating;","Left Shift of the BK Channel; Basilar Artery Myocytes; Molecular Cloning; Heterologous Express; Patch-Clamp Electrophysiology;"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/269"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["We examined the major possible mechanisms for the left shift of the BK channel I-V relationship in native basilar artery myocytes from the two LTH groups. These mechanisms included: differential expression of the accessary BK -1 subunit; differential phosphorylation of the BK subunit; and splice variation of the BK subunit. Using molecular cloning, heterologous expression, and patch-clamp electrophysiology techniques, we elucidated a mechanism that, at least in part, contributes to the differences we observed between channels from native normoxic and LTH myocytes.
"]},{"key":"dc:title","label":"Title","values":["Acclimatization to High-Altitude, Long-Term Hypoxia Alters BK Channel Structure and Function"]}]}],"canonical_facts":{"dc:contributor":["Hessinger, David A.","Buchholz, John N.","Longo, Lawrence D.","Mohan, Subburaman","Watts, Kylie J.","Zhang, Lubo"],"dc:creator":["Tao, Xiaoxiao"],"dc:description.abstract":["We examined the major possible mechanisms for the left shift of the BK channel I-V relationship in native basilar artery myocytes from the two LTH groups. These mechanisms included: differential expression of the accessary BK -1 subunit; differential phosphorylation of the BK subunit; and splice variation of the BK subunit. Using molecular cloning, heterologous expression, and patch-clamp electrophysiology techniques, we elucidated a mechanism that, at least in part, contributes to the differences we observed between channels from native normoxic and LTH myocytes.
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/269"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Chemical and Pharmacologic Phenomena","Medical Pharmacology","Medicine and Health Sciences","Anoxia; Hypoxia-Ischemia - Brain; Acclimatization; Altitude Sickness; Adaptation - Physiological; Myocytes; Potassium Channels - Calcium-Activated; Large-Conductance Calcium-activated Potassium Channels; Calcium Channel Blockers; Ion Channel Gating;","Left Shift of the BK Channel; Basilar Artery Myocytes; Molecular Cloning; Heterologous Express; Patch-Clamp Electrophysiology;"],"dc:title":["Acclimatization to High-Altitude, Long-Term Hypoxia Alters BK Channel Structure and Function"],"thesis:degree_discipline":["Basic Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T02:52:27Z"}