Molecular Mechanisms That Govern Human Cardiac Stem Cell Age Disparity
Transplantation of adult endogenous cardiovascular progenitor cells for heart repair results in some clinical benefit, however these stem cells lack the regenerative capacity unique to neonatal cardiovascular stem cells. The goal of this work was to identify mechanisms that contribute to the decline of cardiac stem cell regenerative ability with age and investigate novel therapeutic strategies to improve cardiac stem cell function. When comparing neonatal and adult cardiovascular stem cell clones, both cell types were capable of cardiomyogenic differentiation. However, the expression levels of forty-one microRNAs were significantly altered with age. Expression differences were correlated with reduced proliferation and a limited capacity to invade in response to growth factor stimulation in adult progenitors. This information can be utilized to develop novel therapeutics and improve outcomes after stem cell-based treatment for heart failure.
Medical Genetics, Medical Microbiology, Medical Molecular Biology, Medicine and Health Sciences, Heart - Physiology; Heart Ventricles; Ventricular Remodeling; Stem Cells; Human Embryonic Stem Cells; Mesenchymal Stromal Cells; Cell aging; Subcellular Fractions; Myocardial Infarction; Cardiac Myosins; Mitogen-Activated Protein Kinase; DNA-Binding Proteins; von Willebrand Factor; Vascular Endothelial Growth Factor A; Gene Expression Regulation, Adult Endogenous Cardiovascular Progenitor Cells; Cell Regenerative Capacity; Cell aging; Neonatal Cardiovascular Stem Cells; Cardiomyogenic Differentiation;
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