HPV16 E6* Induces Oxidative Stress and DNA Damage

<p>High risk types of Human Papillomavirus are the causative agents of virtually all cases of cervical cancer, 50-90% of other anogenital cancers and approximately 30% of oral and pharyngeal cancers. The high-risk types encode two viral oncogenes, E6 and E7, which work together to initiate cell transformation. The approximately 50 amino acid product of the E6* transcript is expressed during the early stages of HPV infection. In this study, we found that expression of E6* increased the level of reactive oxygen species (ROS) in both HPV+ and HPV- cells. This increased oxidative stress led to higher levels of DNA damage. The observed increase in ROS may be due to a decrease in cellular anti-oxidant activity, as we found that E6* expression also led to decreased expression of SOD and Gpx, These studies indicate that E6* may play an important role in virus-induced mutagenesis by increasing oxidative stress and DNA damage.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Basic Sciences

Year

2014

Author dc:creator

• Williams, Vonetta M.

Contributors dc:contributor

• Duerksen-Hughes, Penelope J.
• Brantly, Eileen
• Payne, Kimberly J.
• Reeves, Mark E.
• Soto-Wegner, Ubaldo

• Biochemistry
• Human Papillomavirus 16; Papillomavirus Infections; Uterine Cervical Neoplasms; Mouth Neoplasms
• Human Papillomavirus 16
• Cervical Cancer
• Anogenital Cancer
• Oral and Pharyngeal Cancers
• Virus-Induced Mutagenesis
• Oxidative Stress
• DNA Damage

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/171

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-1170