Untersuchungen zur Eignung von Empfängertieren im Rahmen des Embryotransfers
type: CreativeWork
description: The aim of this study was to test the possibilities of ultrasonography in the examination of recipient animals during commercial embryo transfer in cattle. Altogether 257 animals have been synchronised by a single or a double injection of Prostaglandine F2alpha, 33 potential recipients showed spontaneous heat. On 200 of the animals actually being used as embryo recipients, a rectal palpation as well as an ultrasonographic examination of the ovaries was performed on the day of the transfer and on day 21 of the cycle. The recipients were examined again for pregnancy by ultrasound on day 35 (28 days after the transfer) and by rectal palpation on day 49. A blood sample (plasma) was taken on day 0 (oestrus), 7 (transfer) and 21, in order to mesure the progesterone concentration (P4). An enzyme-based immunological assay was used to evaluate the plasma samples for P4 concentration. The ultrasonographically defined area of the largest cross-section of the corpus luteum was significantly correlated (r = 0,22 ; p < 0,001) with the corpus luteum size evaluated by rectal palpation. There was no relation between the scoring of the corpora lutea performed by palpation and the average P4- concentration (r = 0,13 ; p > 0,05), whereas on day 7 the luteal tissue area measured by ultrasonography was significantly correlated with the P4 values (r = 0,40 ; p < 0,001). In 55 % of the recipients, a sonographically detectable cavity within the corpus luteum was found on the day of transfer. Corpora lutea with a cavity were bigger than those without ( 3,8 cm2 vs. 3 cm2; p < 0,001), however the luteal tissue area of corpora lutea with or without cavity was not significantly different (p > 0,05). On day 21, 136 animals out of 200 (69 %) having received an embryo, showed no signs of oestrus. On day 35 and 49 pregnancy was diagnosed in 50 % and 47 % respectively. The highest pregnancy rate (60 %) was obtained in the group of recipients, with the best score evaluated by rectal palpation. However…
abstract: The aim of this study was to test the possibilities of ultrasonography in the examination of recipient animals during commercial embryo transfer in cattle. Altogether 257 animals have been synchronised by a single or a double injection of Prostaglandine F2alpha, 33 potential recipients showed spontaneous heat. On 200 of the animals actually being used as embryo recipients, a rectal palpation as well as an ultrasonographic examination of the ovaries was performed on the day of the transfer and on day 21 of the cycle. The recipients were examined again for pregnancy by ultrasound on day 35 (28 days after the transfer) and by rectal palpation on day 49. A blood sample (plasma) was taken on day 0 (oestrus), 7 (transfer) and 21, in order to mesure the progesterone concentration (P4). An enzyme-based immunological assay was used to evaluate the plasma samples for P4 concentration. The ultrasonographically defined area of the largest cross-section of the corpus luteum was significantly correlated (r = 0,22 ; p < 0,001) with the corpus luteum size evaluated by rectal palpation. There was no relation between the scoring of the corpora lutea performed by palpation and the average P4- concentration (r = 0,13 ; p > 0,05), whereas on day 7 the luteal tissue area measured by ultrasonography was significantly correlated with the P4 values (r = 0,40 ; p < 0,001). In 55 % of the recipients, a sonographically detectable cavity within the corpus luteum was found on the day of transfer. Corpora lutea with a cavity were bigger than those without ( 3,8 cm2 vs. 3 cm2; p < 0,001), however the luteal tissue area of corpora lutea with or without cavity was not significantly different (p > 0,05). On day 21, 136 animals out of 200 (69 %) having received an embryo, showed no signs of oestrus. On day 35 and 49 pregnancy was diagnosed in 50 % and 47 % respectively. The highest pregnancy rate (60 %) was obtained in the group of recipients, with the best score evaluated by rectal palpation. However…
author: Vaessen, Christiane
date published: 2003-02-07
date modified: 2026-07-24T02:51:38Z
provider: Ludwig Maxmilians Universität München
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Ludwig-Maximilians-Universität