Nachweis und Differenzierung von Vibrio sp. in Krusten- und Schalentieren und Erzeugnissen daraus unter besonderer Berücksichtigung der routinemäßigen Diagnostik

Detection and Differentiation of Vibrio sp. in Crustaceans, Molluscs, and Products thereof with Regard to Routine Diagnostic The purpose of this study was to reveal the contamination rate by vibrios of commercially available crustaceaens, and molluscs, and products thereof. Beside this, a simple quick routine method for the detection should be established. Between January 2001 and May 2002 the administrative „Wirtschaftskontrolldienst“ sampled in the district of Karlsruhe 100 samples of shrimps and shells either raw or heat treated, but not ready to eat, besides this heated and ready to eat food samples. These samples were tested by means of one enrichment system and two bacterial counting methods for Vibrio sp. The enrichment system was modified from the enrichment system described by ICMSF in „Microorganisms in foods – microbiological specifications of food pathogens". The modification is explained thoroughly. Strains of V. parahaemolyticus and V. cholerae were tested afterwards at the “Nationales Referenzzentrum für Enteritiserreger” in Hamburg for the existence of pathogenic potential. A total of 22 percent of the samples were positive for Vibrio; raw shells have the highest contamination rate with 50 percent, raw shrimps (35 % positive) distinctly higher than boiled shrimps (6,8 % positive). Most of the positive results succeeded using the enrichment system. The advantages of the different investigation systems are explained in detail. A potential health risk exist preferably, if improper handling of these seafood leads to an increase of the microbiological load and no or insufficient decontamination prior to eating was performed. Regarding new habits of consumption (raw sea products) and the increase of so called „other forms“ of infectious enteritis more attention should be paid to the sporadic occurrence of this microorganisms.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Ludwig-Maximilians-Universität

Year

2003

Author dc:creator

• Sieffert, Monika

Repository record source_url

https://edoc.ub.uni-muenchen.de/963/

OAI identifier oai:identifier

oai:edoc.ub.uni-muenchen.de:963