Das ovariektomierte und glukokortikoid-behandelte Schaf als Großtiermodell für die Osteoporoseforschung

The aim of this study was to evaluate and establish a large animal model of osteoporosis by a combination of ovariectomy and steroid treatment. A variety of densitometric, morphometric and biomechanical methods were used to quantify changes in ovine bone under estrogen-deficiency and glucocorticoid treatment. With DXA we monitored the progression of the loss of bone mineral density of integral bone at the central and the load-bearing skeleton. Peripheral QCT was employed to determine the different respons es of trabecular and integral bone. High-resolution µCT-images allowed the evaluation of precise morphometrical parameters. Histomorphometric analyses provided information about newly formed osteoid. Stiffness and strength of trabecular bone were assessed by uniaxial compression tests. For all these examinations we utilized integral or trabecular bone from various skeletal locations. The results of this study showed that a combination of ovariectomy and glucocorticoid treatment leads to osteopenia in sh e ep. Bone mineral density of integral bone was reduced during glucocorticoid treatment and returned to normal values thereafter. However, loss of bone mineral at trabecular sites was not reversible. Corticoid treatment also affected the microarchitecture of trabecular bone: bone volume, bone surface and trabecular thickness differed from the control group. However, trabecular number did not change under steroid treatment. Bone stiffness and strength of trabecular bone of corticoid treated animals were m arkedly reduced. These results showed the suitability of the ovariectomized and glucocorticoid treated sheep as an animal model in osteoporosis research. It could be used to develop new orthopaedic techniques or to test prosthetic designs or implants for the treatment of osteoporotic fractures.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Ludwig-Maximilians-Universität

Year

2002

Author dc:creator

• Schorlemmer, Sandra

Repository record source_url

https://edoc.ub.uni-muenchen.de/576/

OAI identifier oai:identifier

oai:edoc.ub.uni-muenchen.de:576