Bestimmung der Vitamin D- Aktivität von calcinogenen Pflanzen im Wachteleischalentest

Determination of vitamin D- activity of calcinogenic plants in the Japanese quail eggshell test In this studie the biological activity of various preparations of the calcinogenic plants Solanum glaucophyllum and Trisetum flavescens is analysed by means of the Japanese quail eggshell test. As main parameters for the evaluation of the biological activity served on the one hand the amount of eggshell dryweight excreted per animal per day as a indicator for the calcium excretion via the eggshell, on the other hand the serum alkaline phosphatase and the content of realitve boneash. Furthermore, additional egg-, bone- and blood parameters were determined. In the two test series which lasted 21 days each vitamin D depleted quails (Coturnix coturnix japonica) were utilized as test animals. In both test series three vitamin D3–standard ratios (100 IU, 200 IU und 400 IU/kg diet) and the metabolite 1,25(OH)2D3 in different concentrations were used. The preparations of Solanum glaucophyllum to be tested was a watersoluble raw extract, an enriched pure extract, a hydrolyzed preparation and preparations of stems and leaves and a preparation of Trisetum flavescens. The influence of glucosidase on the test parameters was analyzed too. The existing linear relationship between vitamin D3-concentration in the diet of the ovendry mass of the eggshells per animal and day forms the basis of the biological activity of the preparations examined.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Ludwig-Maximilians-Universität

Year

2004

Author dc:creator

• Greissinger, Christina

Repository record source_url

https://edoc.ub.uni-muenchen.de/2474/

OAI identifier oai:identifier

oai:edoc.ub.uni-muenchen.de:2474