In situ Metabolism and Biogeography of Phototrophic Consortia
type: CreativeWork
description: >>Novel bacteriochlorophyll e structures and species-specific variability of pigment composition in green sulfur bacteria<< The relative composition of bacteriochlorophyll (BChl) homologs in five different strains of brown-colored green sulfur bacteria was investigated by HPLC-MS/MS and NMR analyses. In addition, the effect of incubation light intensities on homolog distribution was studied in one of the strains (strain Dagow III). A total of 23 different BChl e structures were detected and comprise four homologous porphyrin ring systems and eight different esterifying alcohols. Several BChl e structures are novel. These include a C-8 ethyl, C-12 methyl [E,M] BChl eF homolog which was identified by 1H-NMR analyses of the isolated, main farnesyl homologs (BChl eF). In addition, five previously unknown homolog series with dodecanol, pentadecenol, tetradecanol, hexadecenol and phytol as the esterifying alcohols were detected. The composition of BChl e homologs from the five strains of green sulfur bacteria differed with respect to the relative abundance of the homologs (BChl eF: 25.6 to 67.0% of total BChl e content in stationary cultures). In strain Dagow III, the abundance of BChl eF homologs decreased upon entry into the stationary phase. In all free-living strains, the abundance of BChl eF was increased when the relative carotenoid content was low. The present results provide a more detailed picture of pigment composition in chlorosomes and thus will help to elucidate their structure and function. Furthermore, the newly discovered BChl e molecules are valuable biomarkers for the study of the occurrence and the metabolism of green sulfur bacteria in past and present ecosystems. >>Characterization and in situ carbon metabolism of phototrophic consortia<< A dense population of the phototrophic consortium "P. roseum" was investigated in the chemocline of a temperate holomictic lake (Lake Dagow, Brandenburg, Germany). Fluorescent in situ hybridization revealed that the …
abstract: >>Novel bacteriochlorophyll e structures and species-specific variability of pigment composition in green sulfur bacteria<< The relative composition of bacteriochlorophyll (BChl) homologs in five different strains of brown-colored green sulfur bacteria was investigated by HPLC-MS/MS and NMR analyses. In addition, the effect of incubation light intensities on homolog distribution was studied in one of the strains (strain Dagow III). A total of 23 different BChl e structures were detected and comprise four homologous porphyrin ring systems and eight different esterifying alcohols. Several BChl e structures are novel. These include a C-8 ethyl, C-12 methyl [E,M] BChl eF homolog which was identified by 1H-NMR analyses of the isolated, main farnesyl homologs (BChl eF). In addition, five previously unknown homolog series with dodecanol, pentadecenol, tetradecanol, hexadecenol and phytol as the esterifying alcohols were detected. The composition of BChl e homologs from the five strains of green sulfur bacteria differed with respect to the relative abundance of the homologs (BChl eF: 25.6 to 67.0% of total BChl e content in stationary cultures). In strain Dagow III, the abundance of BChl eF homologs decreased upon entry into the stationary phase. In all free-living strains, the abundance of BChl eF was increased when the relative carotenoid content was low. The present results provide a more detailed picture of pigment composition in chlorosomes and thus will help to elucidate their structure and function. Furthermore, the newly discovered BChl e molecules are valuable biomarkers for the study of the occurrence and the metabolism of green sulfur bacteria in past and present ecosystems. >>Characterization and in situ carbon metabolism of phototrophic consortia<< A dense population of the phototrophic consortium "P. roseum" was investigated in the chemocline of a temperate holomictic lake (Lake Dagow, Brandenburg, Germany). Fluorescent in situ hybridization revealed that the …
author: Glaeser, Jens
date published: 2004-02-12
date modified: 2026-07-24T02:51:53Z
provider: Ludwig Maxmilians Universität München
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Ludwig-Maximilians-Universität