Chemokinrezeptorexpression auf T-Zellen in bronchoalveolärer Lavage und peripherem Blut bei Kindern mit chronischer Bronchitis und interstitiellen Lungenerkrankungen

Background: Lymphocytes are recruited to sites of inflammation by chemokines. Accordingly, a number of chemokine receptors are differentially expressed on effector T cells. We hypothesized that selected T cells accumulate in inflammatory lung diseases involving different pulmonary compartments. To test this hypothesis the frequencies of chemokine receptor expressing T cells were compared in peripheral blood (PB) and bronchoalveolar lavage fluid (BALF) of children with chronic bronchitis and interstitial lung diseases. Methods: BAL was performed in 70 children. According to clinical, macroscopic and cytological findings 37 children were selected for the study and classified as chronic bronchitis (CB, n=17, m=7, mean age 6.6 yrs.) or interstitial lung diseases (ILD, n=20, m=13, mean age 7.0 yrs.). Patients (n=33) with other diagnoses or without cells in BALF were excluded. CD4+ and CD8+ T cells were analyzed in PB (n=30) and BALF (n=37) by flow cytometry. The percentages of CCR5+and CXCR3+ cells were determined within each T cell subset. Results are expressed as medians. For statistical analyses non-parametric tests (Wilcoxon, Mann-Whitney U) were applied. Results: In peripheral blood, the percentage of CXCR3+ T cells (16.4%, range: 0-35.2%) was higher than the percentage of CCR5+ T cells (3.9%, range: 0-19.1%; p<0.001). The percentages of CCR5+ and CXCR3+ PB T cells did not differ between the patient groups. The proportions of CCR5+CD4+ and CCR5+CD8+ cells were clearly higher in BAL (7.8% and 12.4%) than in PB (1.6% and 2.3%) of all patients (p<0.001). The percentage of BAL CXCR3+CD8+ cells was significantly higher in patients with CB (25.2%, range: 0-74.6%) compared to patients with ILD (1.2%, range: 0-16.7%; p<0.05). Conclusions: The results indicate that CCR5+ T cells are a regular constituent of the bronchoalveolar space in pediatric pulmonary diseases. The high frequency of pulmonary CCR5+ T cells may be due to a specific recruitment or alternatively to a local expansion of these cells. Furthermore, CXCR3+CD8+ T cells in bronchoalveolar lavage seem to be characteristic for chronic bronchitis in children.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Ludwig-Maximilians-Universität

Year

2003

Author dc:creator

• Hartl, Dominik

Repository record source_url

https://edoc.ub.uni-muenchen.de/1531/

OAI identifier oai:identifier

oai:edoc.ub.uni-muenchen.de:1531