Nachweis von Salmonella spp. bei Landschildkröten
type: CreativeWork
description: In the present study an efficient procedure for detecting salmonellas in feces of tortoises should be established. First of all a survey of occurrence of salmonellas in reptiles and their importance for reptiles as well as for man is given. Next an appropriate incubation temperature for multiplying salmonellas of reptilian origin in a selective enrichment medium was evaluated. Furthermore 86 salmonella strains isolated from reptiles were tested on nine different selec-tive solid media for their appearance; these media were BPLS-, DCLS-, HE-, MLCB-, ÖNÖZ-, RAMBACH-, SS-, WS- and XLD-Agar. The media were reviewed according to their qualification in identifying those strains. Finally 167 fecal samples of tortoises of different species and two lizards from Munich area were examined according to the former established procedure whether or not they harboured salmonellas. Therewith the suitability of this method should be determined and the incidence of salmonellas of these tortoises should be investigated. Following results were obtained: The best growth of salmonellas resulted from RAPPAPORT-VASSILIADIS and selenite cystine enrichment broth at an incubation temperature of 35-37 °C. The higher critical temperature for incubation of RAPPAPORT-VASSILIADIS enrichment medium is 42 °C; especially Salmonella subspecies IIIb often are inhibited in this medium at this temperature. For isolating salmonellae as well as for inhibiting competing organisms, an incubation temperature of 41 °C is emphasized for enrichment in RAPPAPORT-VASSILIADIS broth. Neither temperature investigated had any inhibitory effect on the growth rate of salmonellas in selenite cystine medium, but its inhibitory activity on competing organisms proved strik-ingly less, too. RAPPAPORT´s enrichment medium supports growing of salmonellas of reptilian origin least of all. For isolation of salmonellas from tortoises BPLS- and XLD-agar proved best for direct strik-ing as well as after previous enrichment in either …
abstract: In the present study an efficient procedure for detecting salmonellas in feces of tortoises should be established. First of all a survey of occurrence of salmonellas in reptiles and their importance for reptiles as well as for man is given. Next an appropriate incubation temperature for multiplying salmonellas of reptilian origin in a selective enrichment medium was evaluated. Furthermore 86 salmonella strains isolated from reptiles were tested on nine different selec-tive solid media for their appearance; these media were BPLS-, DCLS-, HE-, MLCB-, ÖNÖZ-, RAMBACH-, SS-, WS- and XLD-Agar. The media were reviewed according to their qualification in identifying those strains. Finally 167 fecal samples of tortoises of different species and two lizards from Munich area were examined according to the former established procedure whether or not they harboured salmonellas. Therewith the suitability of this method should be determined and the incidence of salmonellas of these tortoises should be investigated. Following results were obtained: The best growth of salmonellas resulted from RAPPAPORT-VASSILIADIS and selenite cystine enrichment broth at an incubation temperature of 35-37 °C. The higher critical temperature for incubation of RAPPAPORT-VASSILIADIS enrichment medium is 42 °C; especially Salmonella subspecies IIIb often are inhibited in this medium at this temperature. For isolating salmonellae as well as for inhibiting competing organisms, an incubation temperature of 41 °C is emphasized for enrichment in RAPPAPORT-VASSILIADIS broth. Neither temperature investigated had any inhibitory effect on the growth rate of salmonellas in selenite cystine medium, but its inhibitory activity on competing organisms proved strik-ingly less, too. RAPPAPORT´s enrichment medium supports growing of salmonellas of reptilian origin least of all. For isolation of salmonellas from tortoises BPLS- and XLD-agar proved best for direct strik-ing as well as after previous enrichment in either …
author: Schramme, Karola Christine
date published: 2003-07-18
date modified: 2026-07-24T02:51:44Z
provider: Ludwig Maxmilians Universität München
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Ludwig-Maximilians-Universität