Novel applications of flow cytometry to assess whole murine skeletal muscle and myonuclei number responses to atrophy and recovery.

This thesis will present an introduction to cellular and molecular adaptation to loading and unloading of skeletal muscle, whilst exploring the advantages and disadvantages of current techniques employed in the field of exercise biochemistry. In response to the current techniques used to assess the signal transduction and protein changes present in response to loading and unloading, we propose a potential, novel method of assessing whole, murine skeletal muscle protein isoforms via flow cytometry. In addition to this, this thesis also explores the changes in morphology and myonuclear number within skeletal muscle following varying periods of atrophy, and recovery from atrophy produced in response to tetrodotoxin administration to silence a motor nerve and therefore muscular contraction in the ankle dorsiflexor muscles of the rat hindlimb.

Name dc:type.qualificationname

mphil

Level dc:type.qualificationlevel

doctoral

Grantor dc:publisher.institution

Liverpool John Moores University

Year dc:date.issued

2018

Author dc:creator

• Viggars, M

Contributors dc:contributor

• Jarvis, J

• QP Physiology
• RC1200 Sports Medicine

DOI dc:identifier.doi

https://doi.org/10.24377/researchonline.ljmu.ac.uk.00009368

OAI identifier oai:identifier

oai:researchonline.ljmu.ac.uk:9368