Mechanisms of pathological adipogenesis
type: CreativeWork
description: Although adipose tissue plays important physiological roles, the increase in adiposity with aging is a common feature in several pathologies, including some forms of osteoporosis. Ion channels and ghrelin signalling have been shown to be involved in regulating many cell processes such as proliferation, differentiation and apoptosis, particularly in bone. This thesis investigated the roles of ghrelin and ion channels in the adipogenic differentiation of 7F2 preosteoblastic cells. The amount of Oil Red O stain increased by 135%, and the mRNA expression of two adipocytic markers, PPARγ (5.46 ± 0.22; p = 0.024) and C/EBPα (25.3 ± 0.21; p = 0.0048), was higher in 7F2 cells cultured with adipogenic medium compared with basal medium, indicating that 7F2 cells differentiated into adipocytes. 7F2 cells also expressed previously unreported variant isoforms of ghrelin at the mRNA level; these variants isoforms were much more abundant than native ghrelin. 7F2 cells also express the other key genes from ghrelin signalling, i.e. Ghsr and Mboat4, suggesting that ghrelin or its variant isoforms may have autocrine/paracrine effects in these cells. Ghrelin mRNA expression was higher in 7F2 cells treated with basal medium + 200 nM ghrelin (144.84 ± 0.58) and adipogenic medium + 200 nM ghrelin (123.07 ± 0.33) compared with cells cultured with basal medium alone (p = 0.01), and adipogenic medium alone (0.79 ± 0.40; p = 0.0089), respectively. Treatment with 20 nM and 200 nM ghrelin had no significant effect on lipid content and on osteoblastic and adipocytic markers mRNA levels (p > 0.05). On the contrary, cell numbers were lower in 7F2 cells cultured with the voltage-gated K+ channel blocker tetraethylammonium, and lipid droplets inside 7F2 cell-derived adipocytes were smaller compared with cells cultured with adipogenic medium alone, suggesting that voltage-gated K+ channels are involved in regulating cell proliferation and adipogenic differentiation. 7F2 cells and 7F2 cell-derived adi…
abstract: Although adipose tissue plays important physiological roles, the increase in adiposity with aging is a common feature in several pathologies, including some forms of osteoporosis. Ion channels and ghrelin signalling have been shown to be involved in regulating many cell processes such as proliferation, differentiation and apoptosis, particularly in bone. This thesis investigated the roles of ghrelin and ion channels in the adipogenic differentiation of 7F2 preosteoblastic cells. The amount of Oil Red O stain increased by 135%, and the mRNA expression of two adipocytic markers, PPARγ (5.46 ± 0.22; p = 0.024) and C/EBPα (25.3 ± 0.21; p = 0.0048), was higher in 7F2 cells cultured with adipogenic medium compared with basal medium, indicating that 7F2 cells differentiated into adipocytes. 7F2 cells also expressed previously unreported variant isoforms of ghrelin at the mRNA level; these variants isoforms were much more abundant than native ghrelin. 7F2 cells also express the other key genes from ghrelin signalling, i.e. Ghsr and Mboat4, suggesting that ghrelin or its variant isoforms may have autocrine/paracrine effects in these cells. Ghrelin mRNA expression was higher in 7F2 cells treated with basal medium + 200 nM ghrelin (144.84 ± 0.58) and adipogenic medium + 200 nM ghrelin (123.07 ± 0.33) compared with cells cultured with basal medium alone (p = 0.01), and adipogenic medium alone (0.79 ± 0.40; p = 0.0089), respectively. Treatment with 20 nM and 200 nM ghrelin had no significant effect on lipid content and on osteoblastic and adipocytic markers mRNA levels (p > 0.05). On the contrary, cell numbers were lower in 7F2 cells cultured with the voltage-gated K+ channel blocker tetraethylammonium, and lipid droplets inside 7F2 cell-derived adipocytes were smaller compared with cells cultured with adipogenic medium alone, suggesting that voltage-gated K+ channels are involved in regulating cell proliferation and adipogenic differentiation. 7F2 cells and 7F2 cell-derived adi…
author: Bastien, M
keywords: QP Physiology, RM Therapeutics. Pharmacology
date published: 2019-06
date modified: 2026-07-24T06:30:28Z
provider: Liverpool Jon Moores University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Liverpool John Moores University

PropertyValue
property ID: DOI
value: 10.24377/LJMU.t.00010739