Genetic polymorphism of the human apolipoproteins A and E

The technique of horizontal, thin layer isoelectric focusing was used to demonstrate the polymorphism of the apolipoproteins E, A-I, A-II and A-IV. Polymorphic forms of apo E were detected after ultracentrifugation of plasma, delipidation of VLDL and isoelectric focusing of the remaining protein. The six common phenotypes, homozygotes E 2/2, E 3/3, E 4/4 and the heterozygotes E 2/3, E 2/4, E 3/4, of apo E were demonstrated. A simplified one step screening method, not requiring ultracentrifugation techniques, was used to demonstrate the A apolipoproteins. As the method uses native serum, epo A-IV was detected as well as apo A-I and apo A-II. The one step screening technique demonstrated reduced levels of apo A-I and A-II in a patient homozygous for Tangier disease. It was also utilised to screen patients undergoing cardiac catheterisation, no variants of apo A-I and A-II were detected in this patient population. The presence of a minor apo A-IV variant in these patients requires confirmation by other techniques.

Grantor dc:publisher

Lincoln College, University of Canterbury

Year dc:date

1985

Author dc:creator

• Joyce, Steve

• genetic polymorphism
• apolipoproteins
• Tangier disease
• lipid disorders
• plasma lipoproteins
• lipoprotein cholesterol
• genetic risk factors
• apolipoprotein A
• apolipoprotein E
• very low density lipoprotein (VLDL)
• ANZSRC::110104 Medical Biochemistry: Lipids
• ANZSRC::110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)

Statement dc:rights

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Licence

• https://researcharchive.lincoln.ac.nz/pages/rights

Language dc:language

en

Identifier

Q112847360

OAI identifier oai:identifier

oai:researcharchive.lincoln.ac.nz:10182/2408