https://search.dev.ndltd.org/etd/lincoln/oai:researcharchive.lincoln.ac.nz:10182/13833
type: CreativeWork
name: Genetics of disease resistance: Molecular characterisation of the major histocompatibility Complex Class II genes in Bos species of different origin : A thesis submitted in partial fulfilment of the requirements for the Degree of Master of Science at Lincoln University
description: Breeding for genetic resilience in farm animals is becoming more important as a consequence of the increasing awareness of pathogen resistance to antibiotics and the need to build more resilient and sustainable agricultural production systems. In this context, the important role the Major Histocompatibility Complex (MHC) proteins play in immune responses would suggest that improved understanding of the MHC genes is vital to understanding and manipulating genetic resilience to infectious diseases. The genes of MHC are highly polymorphic, and studies have suggested that the large variation existing within the MHC region may be attributed to response to selective pressure from pathogens. It has therefore been suggested that the alleles of MHC genes carried by an individual animal may be influenced by the type of pathogens in its environment. An understanding of the allelic variation in MHC genes across multiple geographic regions and breeds could therefore assist us to better understand the influence of selective forces and to better characterise distinct populations of cattle. In this study, four breeds of cattle including White Fulani (n =24), Red Bororo (n =5) and Holstein-White x Fulani-cross (n = 11) from Nigeria, and New Zealand Holstein-Friesian x Jersey-cross (n = 40) were genotyped for variation in exon 2 of BoLA-DQA1. In addition: Yak (n= 47); 13 breeds of cattle including White Fulani (n = 30), Red Bororo (n= 20), and White Fulani × Holstein-cross (n = 16) from Nigeria; Holstein-Friesian × Jersey Cross (n = 22), Hereford (n = 20), Murray Grey (n = 20), Red poll (n = 20), Shorthorn (n =22), Angus (n = 20), Simmental (n = 20), Charolais (n = 20), South Devon (n =20) from New Zealand; and Marchigiana (n = 16) from Italy, were genotyped for variation in exon 2 of BoLA-DRA. A Single Strand Conformation Polymorphism approach was used to genotype the animals and selected cattle were subsequently subjected to direct sequencing of the regions of the genes investigate…
abstract: Breeding for genetic resilience in farm animals is becoming more important as a consequence of the increasing awareness of pathogen resistance to antibiotics and the need to build more resilient and sustainable agricultural production systems. In this context, the important role the Major Histocompatibility Complex (MHC) proteins play in immune responses would suggest that improved understanding of the MHC genes is vital to understanding and manipulating genetic resilience to infectious diseases. The genes of MHC are highly polymorphic, and studies have suggested that the large variation existing within the MHC region may be attributed to response to selective pressure from pathogens. It has therefore been suggested that the alleles of MHC genes carried by an individual animal may be influenced by the type of pathogens in its environment. An understanding of the allelic variation in MHC genes across multiple geographic regions and breeds could therefore assist us to better understand the influence of selective forces and to better characterise distinct populations of cattle. In this study, four breeds of cattle including White Fulani (n =24), Red Bororo (n =5) and Holstein-White x Fulani-cross (n = 11) from Nigeria, and New Zealand Holstein-Friesian x Jersey-cross (n = 40) were genotyped for variation in exon 2 of BoLA-DQA1. In addition: Yak (n= 47); 13 breeds of cattle including White Fulani (n = 30), Red Bororo (n= 20), and White Fulani × Holstein-cross (n = 16) from Nigeria; Holstein-Friesian × Jersey Cross (n = 22), Hereford (n = 20), Murray Grey (n = 20), Red poll (n = 20), Shorthorn (n =22), Angus (n = 20), Simmental (n = 20), Charolais (n = 20), South Devon (n =20) from New Zealand; and Marchigiana (n = 16) from Italy, were genotyped for variation in exon 2 of BoLA-DRA. A Single Strand Conformation Polymorphism approach was used to genotype the animals and selected cattle were subsequently subjected to direct sequencing of the regions of the genes investigate…
author: Oladosu, Oyekunle J.
language: en
keywords: major histocompatibility complex (MHC), nucleotides, BoLA-DQA1, BoLA-DRA, bovine, alleles, polymorphism, genetic markers, single stranded conformational polymorphism, exons, ANZSRC::0604 Genetics, ANZSRC::070201 Animal Breeding, ANZSRC::070705 Veterinary Immunology, ANZSRC::060406 Genetic Immunology
license: https://creativecommons.org/licenses/by-nd/4.0/
date published: 2021
date modified: 2026-07-24T02:50:54Z
provider: Lincoln University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Lincoln University

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property ID: Handle
value: https://hdl.handle.net/10182/13833