Contribution à la compréhension de l'immunopathogénie de l'aspergillose nasosinusale chez le chien
type: CreativeWork
description: Sinonasal aspergillosis is a sever fungal rhinosinusitis mainly affecting large breed dogs in their mid ages. Its most common causal agent is A. fumigatus, a fungus that is largely spread in the atmosphere. As of today, the diagnostic and treatment for this disease remain a challenge for the practicing veterinary doctor. Very little data is available to explain why such a ubiquitous fungus induces a sever rhinosinusitis in otherwise healthy dogs, while other dogs do not present any sign of fungal infection. The authors of a study analysing the expression of mRNA encoding for certain cytokines and chimiokines in the nasal mucosa of SNA affected dogs, propose the assumption that dogs develop a protective immunity (Th1) against A. fumigatus, but that it could be blocked by an excessively intense regulating immunity (massive production of IL-10). Indeed, it is commonly described that in humans affected by invasive aspergillosis, as well as in mice-based models, the production of immunoregulating cytokines (IL-10) should be considered as a sign of the escalation of the sickness and an absence of its remission. The objective of this thesis was to investigate the adaptive immunity reaction of SNA affected dogs based on the assumption that sick subjects develop a protective immunity that is antagonised by a disproportional regulating immunity. Three axes of analysis have been considered to answer this objective. The first looked into the difference of expression and/or production of cytokines and transcriptor factors prototypics of the different adaptative and regulatory immunological paths: Th1 (IFN-γ and Tbet) – Th2 (IL-4 and GATA3) – Th17 (IL-17A and RORc) and Treg (IL-10 and FoxP3) in PBMC of affected and healthy dogs after A. fumigatus stimulation. Secondly, an analysis of genes by microarray has been carried on nasal mucosa biopsies of affected and healthy dogs. Thirdly, the promotor zone of the gene encoding IL-10 in dogs has been analysed by sequencing. This study h…
abstract: Sinonasal aspergillosis is a sever fungal rhinosinusitis mainly affecting large breed dogs in their mid ages. Its most common causal agent is A. fumigatus, a fungus that is largely spread in the atmosphere. As of today, the diagnostic and treatment for this disease remain a challenge for the practicing veterinary doctor. Very little data is available to explain why such a ubiquitous fungus induces a sever rhinosinusitis in otherwise healthy dogs, while other dogs do not present any sign of fungal infection. The authors of a study analysing the expression of mRNA encoding for certain cytokines and chimiokines in the nasal mucosa of SNA affected dogs, propose the assumption that dogs develop a protective immunity (Th1) against A. fumigatus, but that it could be blocked by an excessively intense regulating immunity (massive production of IL-10). Indeed, it is commonly described that in humans affected by invasive aspergillosis, as well as in mice-based models, the production of immunoregulating cytokines (IL-10) should be considered as a sign of the escalation of the sickness and an absence of its remission. The objective of this thesis was to investigate the adaptive immunity reaction of SNA affected dogs based on the assumption that sick subjects develop a protective immunity that is antagonised by a disproportional regulating immunity. Three axes of analysis have been considered to answer this objective. The first looked into the difference of expression and/or production of cytokines and transcriptor factors prototypics of the different adaptative and regulatory immunological paths: Th1 (IFN-γ and Tbet) – Th2 (IL-4 and GATA3) – Th17 (IL-17A and RORc) and Treg (IL-10 and FoxP3) in PBMC of affected and healthy dogs after A. fumigatus stimulation. Secondly, an analysis of genes by microarray has been carried on nasal mucosa biopsies of affected and healthy dogs. Thirdly, the promotor zone of the gene encoding IL-10 in dogs has been analysed by sequencing. This study h…
author: Vanherberghen, Morgane
language: fr
keywords: chien, aspergillose nasosinusale, immunologie, Life sciences, Veterinary medicine & animal health, Sciences du vivant, Médecine vétérinaire & santé animale
license: http://purl.org/coar/access_right/c_16ec
date published: 2014-01-21
date modified: 2026-07-24T02:49:24Z
provider: Université de Liège
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: ULiège - Université de Liège