Production d'oligosaccharides à partir de gomme de caroube
The general framework of this study is the discovery of prebiotics and more specifically the production of oligosaccharides by enzymatic hydrolysis of locust bean gum. These, though not much studied, could present a rich prebiotics potential. The aim of this work was the purification of beta-mannanases located in germinating carob seeds as well as the validation of a AEC-PAD method applicable to the analysis of locust bean gum hydrolysates. Therefore, it has permitted to establish a concentration and purification procedure of beta-mannanases and to determine a suitable preservation method of enzymatic extracts. An original method to evaluate the enzymatic activity by viscosity measurements has also been developed and permits the study of the evolution of enzymatic activities during germination. The validation method of characterization and quantification of (galacto)-manno-oligosaccharides constitutes the last part studied. Seven performance criteria calculated for each of the nine standards were used to validate the method. It has then been used to analyze locust bean gum hydrolysates. The results of this work are the first of a new research theme. They offer many opportunities and future applications.
locust bean gum, enzymatic hydrolysis, prebiotic, gomme de caroube, hydrolyse enzymatique, prébiotique, Physical, chemical, mathematical & earth Sciences, Chemistry, Physique, chimie, mathématiques & sciences de la terre, Chimie
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