{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/6548"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/6548","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"In vitro evaluation of linear plant host defense peptides Shepherin 2, Skh-AMP1, Cn-AMP1, and Cr-ACP1 for cytotoxicity and antimicrobial activities against potato pathogens","abstract":"The aim of this study was to evaluate the antimicrobial and cytotoxic activities of four linear plant host defense peptides (HDPs), Shepherin 2, Cr-ACP1, Skh-AMP1, and Cn-AMP1, in vitro and to identify potential candidates for transgenic expression in potato plants for enhanced disease resistance. The antimicrobial activities of the HDPs were tested against major potato pathogens: the fungi Fusarium culmorum and Alternaria solani, and the bacteria Pectobacterium carotovorum and P. atrosepticum. Shepherin 2 had the strongest antimicrobial activity, inhibiting the germination of 98% F. culmorum conidia at 16 µM, and 84% P. carotovorum cells at 64 µM. Combination of Shepherin 2 with Cr-ACP1 resulted in inhibition of 89% P. carotovorum cells over the expected 70% at 16μM, indicating a synergistic interaction. All HDPs had low cytotoxicities toward plant protoplasts and mammalian cells. The gene encoding Shepherin 2 was introduced into potato plants for future evaluation of its activity in planta.","abstract_html":"The aim of this study was to evaluate the antimicrobial and cytotoxic activities of four linear plant host defense peptides (HDPs), Shepherin 2, Cr-ACP1, Skh-AMP1, and Cn-AMP1, in vitro and to identify potential candidates for transgenic expression in potato plants for enhanced disease resistance. The antimicrobial activities of the HDPs were tested against major potato pathogens: the fungi Fusarium culmorum and Alternaria solani, and the bacteria Pectobacterium carotovorum and P. atrosepticum. Shepherin 2 had the strongest antimicrobial activity, inhibiting the germination of 98% F. culmorum conidia at 16 µM, and 84% P. carotovorum cells at 64 µM. Combination of Shepherin 2 with Cr-ACP1 resulted in inhibition of 89% P. carotovorum cells over the expected 70% at 16μM, indicating a synergistic interaction. All HDPs had low cytotoxicities toward plant protoplasts and mammalian cells. The gene encoding Shepherin 2 was introduced into potato plants for future evaluation of its activity in planta.","abstract_has_math":false,"creators":["Stephen, Christie M.","University of Lethbridge. Faculty of Arts and Science"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023","date_published":"2023","updated_at":"2026-07-27T20:02:11Z","subjects":["linear plant host defense peptides","cytotoxicity activity","antimicrobial activity","potato pathogens","Shepherin 2","Cr-ACP1","Skh-AMP1","Cn-AMP1"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/6548"],"render_values":[{"text":"hdl:10133/6548","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2023"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["linear plant host defense peptides","cytotoxicity activity","antimicrobial activity","potato pathogens","Shepherin 2","Cr-ACP1","Skh-AMP1","Cn-AMP1"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/6548"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.other","label":"Dc Description Other","values":["The aim of this study was to evaluate the antimicrobial and cytotoxic activities of four linear plant host defense peptides (HDPs), Shepherin 2, Cr-ACP1, Skh-AMP1, and Cn-AMP1, in vitro and to identify potential candidates for transgenic expression in potato plants for enhanced disease resistance. The antimicrobial activities of the HDPs were tested against major potato pathogens: the fungi Fusarium culmorum and Alternaria solani, and the bacteria Pectobacterium carotovorum and P. atrosepticum. Shepherin 2 had the strongest antimicrobial activity, inhibiting the germination of 98% F. culmorum conidia at 16 µM, and 84% P. carotovorum cells at 64 µM. Combination of Shepherin 2 with Cr-ACP1 resulted in inhibition of 89% P. carotovorum cells over the expected 70% at 16μM, indicating a synergistic interaction. All HDPs had low cytotoxicities toward plant protoplasts and mammalian cells. The gene encoding Shepherin 2 was introduced into potato plants for future evaluation of its activity in planta."]},{"key":"dc:title","label":"Title","values":["In vitro evaluation of linear plant host defense peptides Shepherin 2, Skh-AMP1, Cn-AMP1, and Cr-ACP1 for cytotoxicity and antimicrobial activities against potato pathogens"]}]}],"canonical_facts":{"dc:date.issued":["2023"],"dc:description.other":["The aim of this study was to evaluate the antimicrobial and cytotoxic activities of four linear plant host defense peptides (HDPs), Shepherin 2, Cr-ACP1, Skh-AMP1, and Cn-AMP1, in vitro and to identify potential candidates for transgenic expression in potato plants for enhanced disease resistance. The antimicrobial activities of the HDPs were tested against major potato pathogens: the fungi Fusarium culmorum and Alternaria solani, and the bacteria Pectobacterium carotovorum and P. atrosepticum. Shepherin 2 had the strongest antimicrobial activity, inhibiting the germination of 98% F. culmorum conidia at 16 µM, and 84% P. carotovorum cells at 64 µM. Combination of Shepherin 2 with Cr-ACP1 resulted in inhibition of 89% P. carotovorum cells over the expected 70% at 16μM, indicating a synergistic interaction. All HDPs had low cytotoxicities toward plant protoplasts and mammalian cells. The gene encoding Shepherin 2 was introduced into potato plants for future evaluation of its activity in planta."],"dc:identifier":["hdl:10133/6548"],"dc:subject":["linear plant host defense peptides","cytotoxicity activity","antimicrobial activity","potato pathogens","Shepherin 2","Cr-ACP1","Skh-AMP1","Cn-AMP1"],"dc:title":["In vitro evaluation of linear plant host defense peptides Shepherin 2, Skh-AMP1, Cn-AMP1, and Cr-ACP1 for cytotoxicity and antimicrobial activities against potato pathogens"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T20:02:11Z"}