{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/6515"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/6515","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"Characterization and rational design of biomolecular sensors using molecular dynamics simulations","abstract":"Biosensors are analytical devices that use biological components to detect and report the presence of a target molecule. Although useful for a broad range of purposes, biosensors are conventionally designed using laborious methods limiting development to a small number of applications with large commercial value. To overcome this limitation, computational approaches are needed to streamline rational design of protein-fluorophore conjugate-type biosensors. Here, I report and iteratively improve such a biosensor development pipeline based on protein molecular dynamics simulations, exploiting underlying dynamic properties of proteins for biosensor design. As proof-of-concept, I report the construction of several carbohydrate-detecting biosensors which are advantageous compared to previous carbohydrate detection methods, and I use these biomolecular tools to characterize several Carbohydrate Active Enzymes (CAZymes). This research highlights how underlying dynamic features of proteins can be utilized for the design and mechanistic interpretation of biomolecular function in a broad range of applications.","abstract_html":"Biosensors are analytical devices that use biological components to detect and report the presence of a target molecule. Although useful for a broad range of purposes, biosensors are conventionally designed using laborious methods limiting development to a small number of applications with large commercial value. To overcome this limitation, computational approaches are needed to streamline rational design of protein-fluorophore conjugate-type biosensors. Here, I report and iteratively improve such a biosensor development pipeline based on protein molecular dynamics simulations, exploiting underlying dynamic properties of proteins for biosensor design. As proof-of-concept, I report the construction of several carbohydrate-detecting biosensors which are advantageous compared to previous carbohydrate detection methods, and I use these biomolecular tools to characterize several Carbohydrate Active Enzymes (CAZymes). This research highlights how underlying dynamic features of proteins can be utilized for the design and mechanistic interpretation of biomolecular function in a broad range of applications.","abstract_has_math":false,"creators":["Smith, Dustin D.","University of Lethbridge. 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Although useful for a broad range of purposes, biosensors are conventionally designed using laborious methods limiting development to a small number of applications with large commercial value. To overcome this limitation, computational approaches are needed to streamline rational design of protein-fluorophore conjugate-type biosensors. Here, I report and iteratively improve such a biosensor development pipeline based on protein molecular dynamics simulations, exploiting underlying dynamic properties of proteins for biosensor design. As proof-of-concept, I report the construction of several carbohydrate-detecting biosensors which are advantageous compared to previous carbohydrate detection methods, and I use these biomolecular tools to characterize several Carbohydrate Active Enzymes (CAZymes). This research highlights how underlying dynamic features of proteins can be utilized for the design and mechanistic interpretation of biomolecular function in a broad range of applications."]},{"key":"dc:description.other","label":"Dc Description Other","values":["Biosensors are analytical devices that use biological components to detect and report the presence of a target molecule. Although useful for a broad range of purposes, biosensors are conventionally designed using laborious methods limiting development to a small number of applications with large commercial value. To overcome this limitation, computational approaches are needed to streamline rational design of protein-fluorophore conjugate-type biosensors. Here, I report and iteratively improve such a biosensor development pipeline based on protein molecular dynamics simulations, exploiting underlying dynamic properties of proteins for biosensor design. As proof-of-concept, I report the construction of several carbohydrate-detecting biosensors which are advantageous compared to previous carbohydrate detection methods, and I use these biomolecular tools to characterize several Carbohydrate Active Enzymes (CAZymes). This research highlights how underlying dynamic features of proteins can be utilized for the design and mechanistic interpretation of biomolecular function in a broad range of applications."]},{"key":"dc:title","label":"Title","values":["Characterization and rational design of biomolecular sensors using molecular dynamics simulations"]}]}],"canonical_facts":{"dc:contributor.supervisor":["Wieden, Hans-Joachim","Patel, Trushar R."],"dc:creator":["Smith, Dustin D.","University of Lethbridge. 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As proof-of-concept, I report the construction of several carbohydrate-detecting biosensors which are advantageous compared to previous carbohydrate detection methods, and I use these biomolecular tools to characterize several Carbohydrate Active Enzymes (CAZymes). This research highlights how underlying dynamic features of proteins can be utilized for the design and mechanistic interpretation of biomolecular function in a broad range of applications."],"dc:description.other":["Biosensors are analytical devices that use biological components to detect and report the presence of a target molecule. Although useful for a broad range of purposes, biosensors are conventionally designed using laborious methods limiting development to a small number of applications with large commercial value. To overcome this limitation, computational approaches are needed to streamline rational design of protein-fluorophore conjugate-type biosensors. Here, I report and iteratively improve such a biosensor development pipeline based on protein molecular dynamics simulations, exploiting underlying dynamic properties of proteins for biosensor design. As proof-of-concept, I report the construction of several carbohydrate-detecting biosensors which are advantageous compared to previous carbohydrate detection methods, and I use these biomolecular tools to characterize several Carbohydrate Active Enzymes (CAZymes). 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