{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/6379"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/6379","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"Engineering cell-free systems for synthetic biologists","abstract":"Synthetic biology (synbio) has emerged as a transformative scientific field with immense potential to address a wide-range of global problems. A specific sub-field of synbio utilizes cellular biomolecular machinery outside of a living cell. In theory, these “cell-free” systems offer a simpler approach and unique features compared to cell-based systems for biotechnology development. However, in practice limited accessibility and poor protein synthesis capacity hinder the overall scope and application of cell-free synbio. To address these challenges, it was our goal to create new engineering tools that will help expand the overall utility of cell-free expression systems. Data presented here provides: 1) detailed methods for the in-house preparation of a cost-effective in vitro reconstituted cell-free system, 2) an in-depth proteomic analysis of the system building blocks as a tool to characterize the composition and inform optimization, and 3) an improvement to protein synthesis capacity by modifying the ribosome composition. Furthermore, a critical assessment of the regulatory landscape is provided, promoting the safe and responsible use of cell-free synbio.","abstract_html":"Synthetic biology (synbio) has emerged as a transformative scientific field with immense potential to address a wide-range of global problems. A specific sub-field of synbio utilizes cellular biomolecular machinery outside of a living cell. In theory, these “cell-free” systems offer a simpler approach and unique features compared to cell-based systems for biotechnology development. However, in practice limited accessibility and poor protein synthesis capacity hinder the overall scope and application of cell-free synbio. To address these challenges, it was our goal to create new engineering tools that will help expand the overall utility of cell-free expression systems. Data presented here provides: 1) detailed methods for the in-house preparation of a cost-effective in vitro reconstituted cell-free system, 2) an in-depth proteomic analysis of the system building blocks as a tool to characterize the composition and inform optimization, and 3) an improvement to protein synthesis capacity by modifying the ribosome composition. Furthermore, a critical assessment of the regulatory landscape is provided, promoting the safe and responsible use of cell-free synbio.","abstract_has_math":false,"creators":["Sheahan, Taylor Anne","University of Lethbridge. Faculty of Arts and Science"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022","date_published":"2022","updated_at":"2026-07-27T20:02:17Z","subjects":["synthetic biology","cell-free systems","Proteins--Synthesis","Proteomics","Biotechnology","Bioengineering","Dissertations, Academic"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/6379"],"render_values":[{"text":"hdl:10133/6379","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2022"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["synthetic biology","cell-free systems","Proteins--Synthesis","Proteomics","Biotechnology","Bioengineering","Dissertations, Academic"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/6379"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.other","label":"Dc Description Other","values":["Synthetic biology (synbio) has emerged as a transformative scientific field with immense potential to address a wide-range of global problems. A specific sub-field of synbio utilizes cellular biomolecular machinery outside of a living cell. In theory, these “cell-free” systems offer a simpler approach and unique features compared to cell-based systems for biotechnology development. However, in practice limited accessibility and poor protein synthesis capacity hinder the overall scope and application of cell-free synbio. To address these challenges, it was our goal to create new engineering tools that will help expand the overall utility of cell-free expression systems. Data presented here provides: 1) detailed methods for the in-house preparation of a cost-effective in vitro reconstituted cell-free system, 2) an in-depth proteomic analysis of the system building blocks as a tool to characterize the composition and inform optimization, and 3) an improvement to protein synthesis capacity by modifying the ribosome composition. Furthermore, a critical assessment of the regulatory landscape is provided, promoting the safe and responsible use of cell-free synbio."]},{"key":"dc:title","label":"Title","values":["Engineering cell-free systems for synthetic biologists"]}]}],"canonical_facts":{"dc:date.issued":["2022"],"dc:description.other":["Synthetic biology (synbio) has emerged as a transformative scientific field with immense potential to address a wide-range of global problems. A specific sub-field of synbio utilizes cellular biomolecular machinery outside of a living cell. In theory, these “cell-free” systems offer a simpler approach and unique features compared to cell-based systems for biotechnology development. However, in practice limited accessibility and poor protein synthesis capacity hinder the overall scope and application of cell-free synbio. To address these challenges, it was our goal to create new engineering tools that will help expand the overall utility of cell-free expression systems. Data presented here provides: 1) detailed methods for the in-house preparation of a cost-effective in vitro reconstituted cell-free system, 2) an in-depth proteomic analysis of the system building blocks as a tool to characterize the composition and inform optimization, and 3) an improvement to protein synthesis capacity by modifying the ribosome composition. Furthermore, a critical assessment of the regulatory landscape is provided, promoting the safe and responsible use of cell-free synbio."],"dc:identifier":["hdl:10133/6379"],"dc:subject":["synthetic biology","cell-free systems","Proteins--Synthesis","Proteomics","Biotechnology","Bioengineering","Dissertations, Academic"],"dc:title":["Engineering cell-free systems for synthetic biologists"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T20:02:17Z"}