{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/6127"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/6127","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"Biophysical characterisation studies of hantaviral and human long noncoding RNAs","abstract":"Emerging zoonotic viruses are a global threat that can cause devastating outbreaks and indelible economic fallout to human systems. North American Hantavirus is a NIAID Category A Priority Pathogen, that regularly involves their Noncoding Terminal Regions to instigate viral replication and translation. Long Noncoding RNAs are integral regulatory molecules involved in a myriad of biological processes. This thesis seeks to identify human RNA-Binding Proteins involved in Hantaviral lifecycles, and to biophysically characterise human Long Noncoding RNAs that mediate cancer progression. Pull-Down Assays identified several RNA stabilising and salient viral lifecycle promoting proteins that putatively interact with Hantaviral Noncoding Terminal Regions. Small Angle X-ray Scattering and coarse-grained SimRNA computational modelling methods generated combined low/high-resolution three-dimensional structures of Long Intergenic Noncoding RNA-p21 Alu Inverted Repeats. Furthermore, we have employed orthogonal biophysical approaches involving Analytical Ultracentrifugation and Multiangle/Dynamic Light Scattering techniques to verify target in vitro transcribed RNA for accuracy.","abstract_html":"Emerging zoonotic viruses are a global threat that can cause devastating outbreaks and indelible economic fallout to human systems. North American Hantavirus is a NIAID Category A Priority Pathogen, that regularly involves their Noncoding Terminal Regions to instigate viral replication and translation. Long Noncoding RNAs are integral regulatory molecules involved in a myriad of biological processes. This thesis seeks to identify human RNA-Binding Proteins involved in Hantaviral lifecycles, and to biophysically characterise human Long Noncoding RNAs that mediate cancer progression. Pull-Down Assays identified several RNA stabilising and salient viral lifecycle promoting proteins that putatively interact with Hantaviral Noncoding Terminal Regions. Small Angle X-ray Scattering and coarse-grained SimRNA computational modelling methods generated combined low/high-resolution three-dimensional structures of Long Intergenic Noncoding RNA-p21 Alu Inverted Repeats. Furthermore, we have employed orthogonal biophysical approaches involving Analytical Ultracentrifugation and Multiangle/Dynamic Light Scattering techniques to verify target in vitro transcribed RNA for accuracy.","abstract_has_math":false,"creators":["D'Souza, Michael H."],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022","date_published":"2022","updated_at":"2026-07-27T20:02:11Z","subjects":["RNA","Biochemistry","Hantavirus","LincRNA-p21 AluSx1","RNA-Binding Proteins","Small Angle X-ray Scattering","Dissertations, Academic","Hantaviruses"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/6127"],"render_values":[{"text":"hdl:10133/6127","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2022"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["RNA","Biochemistry","Hantavirus","LincRNA-p21 AluSx1","RNA-Binding Proteins","Small Angle X-ray Scattering","Dissertations, Academic","Hantaviruses"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/6127"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.other","label":"Dc Description Other","values":["Emerging zoonotic viruses are a global threat that can cause devastating outbreaks and indelible economic fallout to human systems. North American Hantavirus is a NIAID Category A Priority Pathogen, that regularly involves their Noncoding Terminal Regions to instigate viral replication and translation. Long Noncoding RNAs are integral regulatory molecules involved in a myriad of biological processes. This thesis seeks to identify human RNA-Binding Proteins involved in Hantaviral lifecycles, and to biophysically characterise human Long Noncoding RNAs that mediate cancer progression. Pull-Down Assays identified several RNA stabilising and salient viral lifecycle promoting proteins that putatively interact with Hantaviral Noncoding Terminal Regions. Small Angle X-ray Scattering and coarse-grained SimRNA computational modelling methods generated combined low/high-resolution three-dimensional structures of Long Intergenic Noncoding RNA-p21 Alu Inverted Repeats. Furthermore, we have employed orthogonal biophysical approaches involving Analytical Ultracentrifugation and Multiangle/Dynamic Light Scattering techniques to verify target in vitro transcribed RNA for accuracy."]},{"key":"dc:title","label":"Title","values":["Biophysical characterisation studies of hantaviral and human long noncoding RNAs"]}]}],"canonical_facts":{"dc:date.issued":["2022"],"dc:description.other":["Emerging zoonotic viruses are a global threat that can cause devastating outbreaks and indelible economic fallout to human systems. North American Hantavirus is a NIAID Category A Priority Pathogen, that regularly involves their Noncoding Terminal Regions to instigate viral replication and translation. Long Noncoding RNAs are integral regulatory molecules involved in a myriad of biological processes. This thesis seeks to identify human RNA-Binding Proteins involved in Hantaviral lifecycles, and to biophysically characterise human Long Noncoding RNAs that mediate cancer progression. Pull-Down Assays identified several RNA stabilising and salient viral lifecycle promoting proteins that putatively interact with Hantaviral Noncoding Terminal Regions. Small Angle X-ray Scattering and coarse-grained SimRNA computational modelling methods generated combined low/high-resolution three-dimensional structures of Long Intergenic Noncoding RNA-p21 Alu Inverted Repeats. Furthermore, we have employed orthogonal biophysical approaches involving Analytical Ultracentrifugation and Multiangle/Dynamic Light Scattering techniques to verify target in vitro transcribed RNA for accuracy."],"dc:identifier":["hdl:10133/6127"],"dc:subject":["RNA","Biochemistry","Hantavirus","LincRNA-p21 AluSx1","RNA-Binding Proteins","Small Angle X-ray Scattering","Dissertations, Academic","Hantaviruses"],"dc:title":["Biophysical characterisation studies of hantaviral and human long noncoding RNAs"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T20:02:11Z"}