{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/5819"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/5819","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"The fear conditioned response under head fixation","abstract":"In classic measures of fear induced freezing behavior, animals stop all movement except breathing. Many large-scale recording techniques in modern neuroscience such as wide-field and two-photon fluorescent imaging require animals be in a head-fixed preparation. Here we demonstrate that it is possible to measure freezing behavior in head-fixed mice based on video measurements of motion, the pupillary response, and electromyography of neck muscles. Animals were either conditioned to form an association between a tone (conditioned stimulus; CS) and a footshock (unconditioned stimulus; US) or were presented both tones and footshocks explicitly unpaired. Animals who were conditioned to form an association showed less movement, and a stronger pupil response when presented with the CS. We outline key considerations which are likely important in establishing a strong fear response in a head-fixed preparation.","abstract_html":"In classic measures of fear induced freezing behavior, animals stop all movement except breathing. Many large-scale recording techniques in modern neuroscience such as wide-field and two-photon fluorescent imaging require animals be in a head-fixed preparation. Here we demonstrate that it is possible to measure freezing behavior in head-fixed mice based on video measurements of motion, the pupillary response, and electromyography of neck muscles. Animals were either conditioned to form an association between a tone (conditioned stimulus; CS) and a footshock (unconditioned stimulus; US) or were presented both tones and footshocks explicitly unpaired. Animals who were conditioned to form an association showed less movement, and a stronger pupil response when presented with the CS. We outline key considerations which are likely important in establishing a strong fear response in a head-fixed preparation.","abstract_has_math":false,"creators":["Naghizadeh, Milad","University of Lethbridge. Faculty of Arts and Science"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020","date_published":"2020","updated_at":"2026-07-27T20:02:29Z","subjects":["Dissertations, Academic","Fear","Fear in animals","Emotions in animals","Psychology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/5819"],"render_values":[{"text":"hdl:10133/5819","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2020"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Dissertations, Academic","Fear","Fear in animals","Emotions in animals","Psychology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/5819"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.other","label":"Dc Description Other","values":["In classic measures of fear induced freezing behavior, animals stop all movement except breathing. Many large-scale recording techniques in modern neuroscience such as wide-field and two-photon fluorescent imaging require animals be in a head-fixed preparation. Here we demonstrate that it is possible to measure freezing behavior in head-fixed mice based on video measurements of motion, the pupillary response, and electromyography of neck muscles. Animals were either conditioned to form an association between a tone (conditioned stimulus; CS) and a footshock (unconditioned stimulus; US) or were presented both tones and footshocks explicitly unpaired. Animals who were conditioned to form an association showed less movement, and a stronger pupil response when presented with the CS. We outline key considerations which are likely important in establishing a strong fear response in a head-fixed preparation."]},{"key":"dc:title","label":"Title","values":["The fear conditioned response under head fixation"]}]}],"canonical_facts":{"dc:date.issued":["2020"],"dc:description.other":["In classic measures of fear induced freezing behavior, animals stop all movement except breathing. Many large-scale recording techniques in modern neuroscience such as wide-field and two-photon fluorescent imaging require animals be in a head-fixed preparation. Here we demonstrate that it is possible to measure freezing behavior in head-fixed mice based on video measurements of motion, the pupillary response, and electromyography of neck muscles. Animals were either conditioned to form an association between a tone (conditioned stimulus; CS) and a footshock (unconditioned stimulus; US) or were presented both tones and footshocks explicitly unpaired. Animals who were conditioned to form an association showed less movement, and a stronger pupil response when presented with the CS. We outline key considerations which are likely important in establishing a strong fear response in a head-fixed preparation."],"dc:identifier":["hdl:10133/5819"],"dc:subject":["Dissertations, Academic","Fear","Fear in animals","Emotions in animals","Psychology"],"dc:title":["The fear conditioned response under head fixation"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T20:02:29Z"}