{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/5764"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/5764","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"Development of a real time immuno-PCR assay for the detection of pea root rot causal agent, Aphanomyces euteiches","abstract":"Effective measures to detect Aphanomyces euteiches, one of the devastating pathogen of pea, are lacking. An indirect ELISA and a real time immuno-PCR (RT-iPCR) assay for the timely and sensitive detection of A. euteiches were developed using antiserum specific to oospores of A. euteiches isolates. RT-iPCR was 100 times more sensitive over the linear working range than indirect ELISA. To assess the performance of the RT-iPCR assay, non-infested soils representing a range of soil textures were collected from Alberta fields. An extraction protocol was developed by spiking the soils with A. euteiches oospores and the RT-iPCR assay was used to quantify oospores in the soil extracts. The method performed well at the concentration 100 oospores/g and above. A rolled towel bioassay and root PCR were performed on infested field samples which showed a good agreement with our RT-iPCR results. Thus, RT-iPCR may be an invaluable tool for field diagnostics.","abstract_html":"Effective measures to detect Aphanomyces euteiches, one of the devastating pathogen of pea, are lacking. An indirect ELISA and a real time immuno-PCR (RT-iPCR) assay for the timely and sensitive detection of A. euteiches were developed using antiserum specific to oospores of A. euteiches isolates. RT-iPCR was 100 times more sensitive over the linear working range than indirect ELISA. To assess the performance of the RT-iPCR assay, non-infested soils representing a range of soil textures were collected from Alberta fields. An extraction protocol was developed by spiking the soils with A. euteiches oospores and the RT-iPCR assay was used to quantify oospores in the soil extracts. The method performed well at the concentration 100 oospores/g and above. A rolled towel bioassay and root PCR were performed on infested field samples which showed a good agreement with our RT-iPCR results. Thus, RT-iPCR may be an invaluable tool for field diagnostics.","abstract_has_math":false,"creators":["Kaphle, Salina","University of Lethbridge. Faculty of Arts and Science"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020","date_published":"2020","updated_at":"2026-07-27T20:02:32Z","subjects":["Dissertations, Academic","Aphanomyces euteiches","Enzyme-linked immunosorbent assay","Legumes -- Diseases and pests","Root rots"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/5764"],"render_values":[{"text":"hdl:10133/5764","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2020"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Dissertations, Academic","Aphanomyces euteiches","Enzyme-linked immunosorbent assay","Legumes -- Diseases and pests","Root rots"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/5764"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.other","label":"Dc Description Other","values":["Effective measures to detect Aphanomyces euteiches, one of the devastating pathogen of pea, are lacking. An indirect ELISA and a real time immuno-PCR (RT-iPCR) assay for the timely and sensitive detection of A. euteiches were developed using antiserum specific to oospores of A. euteiches isolates. RT-iPCR was 100 times more sensitive over the linear working range than indirect ELISA. To assess the performance of the RT-iPCR assay, non-infested soils representing a range of soil textures were collected from Alberta fields. An extraction protocol was developed by spiking the soils with A. euteiches oospores and the RT-iPCR assay was used to quantify oospores in the soil extracts. The method performed well at the concentration 100 oospores/g and above. A rolled towel bioassay and root PCR were performed on infested field samples which showed a good agreement with our RT-iPCR results. Thus, RT-iPCR may be an invaluable tool for field diagnostics."]},{"key":"dc:title","label":"Title","values":["Development of a real time immuno-PCR assay for the detection of pea root rot causal agent, Aphanomyces euteiches"]}]}],"canonical_facts":{"dc:date.issued":["2020"],"dc:description.other":["Effective measures to detect Aphanomyces euteiches, one of the devastating pathogen of pea, are lacking. An indirect ELISA and a real time immuno-PCR (RT-iPCR) assay for the timely and sensitive detection of A. euteiches were developed using antiserum specific to oospores of A. euteiches isolates. RT-iPCR was 100 times more sensitive over the linear working range than indirect ELISA. To assess the performance of the RT-iPCR assay, non-infested soils representing a range of soil textures were collected from Alberta fields. An extraction protocol was developed by spiking the soils with A. euteiches oospores and the RT-iPCR assay was used to quantify oospores in the soil extracts. The method performed well at the concentration 100 oospores/g and above. A rolled towel bioassay and root PCR were performed on infested field samples which showed a good agreement with our RT-iPCR results. Thus, RT-iPCR may be an invaluable tool for field diagnostics."],"dc:identifier":["hdl:10133/5764"],"dc:subject":["Dissertations, Academic","Aphanomyces euteiches","Enzyme-linked immunosorbent assay","Legumes -- Diseases and pests","Root rots"],"dc:title":["Development of a real time immuno-PCR assay for the detection of pea root rot causal agent, Aphanomyces euteiches"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T20:02:32Z"}