{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/5456"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/5456","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"Development of RNA-based translation modulation tools for synthetic biology applications","abstract":"The ability to reliably regulate translation of heterologous proteins is of great interest for diverse applications in the field of biological and metabolic engineering. RNA-based tools to control ribosome-dependent synthesis of proteins within E. coli were developed. First, adaptation of the well-studied RNA-IN and RNA-OUT system based on E. coli Tn10 to reliably repress translation regardless of coding sequence context was performed. Using an invariant sequence within the RNA-IN/OUT interacting region does not interfere with the function of the RNAs involved. The same RNA-IN/RNA-OUT system was adapted to activate the translation of a specific target mRNA. Regulation of translation extends to processing of polyproteins. In eukaryotes this can be accomplished by viral 2A peptides, avoiding reinitiating at a downstream start codon but has not been observed in bacteria. Therefore, a strategy for developing libraries of 2A peptides with potential activity across an array of organisms was developed.","abstract_html":"The ability to reliably regulate translation of heterologous proteins is of great interest for diverse applications in the field of biological and metabolic engineering. RNA-based tools to control ribosome-dependent synthesis of proteins within E. coli were developed. First, adaptation of the well-studied RNA-IN and RNA-OUT system based on E. coli Tn10 to reliably repress translation regardless of coding sequence context was performed. Using an invariant sequence within the RNA-IN/OUT interacting region does not interfere with the function of the RNAs involved. The same RNA-IN/RNA-OUT system was adapted to activate the translation of a specific target mRNA. Regulation of translation extends to processing of polyproteins. In eukaryotes this can be accomplished by viral 2A peptides, avoiding reinitiating at a downstream start codon but has not been observed in bacteria. Therefore, a strategy for developing libraries of 2A peptides with potential activity across an array of organisms was developed.","abstract_has_math":false,"creators":["Hakstol, Rhys J.","University of Lethbridge. Faculty of Arts and Science"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019","date_published":"2019","updated_at":"2026-07-27T20:02:20Z","subjects":["2A-peptides","biochemistry","gene expression regulation","novel trans-acting RNAs","prokaryotic gene expression","synthetic biology","Dissertations, Academic"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/5456"],"render_values":[{"text":"hdl:10133/5456","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2019"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["2A-peptides","biochemistry","gene expression regulation","novel trans-acting RNAs","prokaryotic gene expression","synthetic biology","Dissertations, Academic"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/5456"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.other","label":"Dc Description Other","values":["The ability to reliably regulate translation of heterologous proteins is of great interest for diverse applications in the field of biological and metabolic engineering. RNA-based tools to control ribosome-dependent synthesis of proteins within E. coli were developed. First, adaptation of the well-studied RNA-IN and RNA-OUT system based on E. coli Tn10 to reliably repress translation regardless of coding sequence context was performed. Using an invariant sequence within the RNA-IN/OUT interacting region does not interfere with the function of the RNAs involved. The same RNA-IN/RNA-OUT system was adapted to activate the translation of a specific target mRNA. Regulation of translation extends to processing of polyproteins. In eukaryotes this can be accomplished by viral 2A peptides, avoiding reinitiating at a downstream start codon but has not been observed in bacteria. Therefore, a strategy for developing libraries of 2A peptides with potential activity across an array of organisms was developed."]},{"key":"dc:title","label":"Title","values":["Development of RNA-based translation modulation tools for synthetic biology applications"]}]}],"canonical_facts":{"dc:date.issued":["2019"],"dc:description.other":["The ability to reliably regulate translation of heterologous proteins is of great interest for diverse applications in the field of biological and metabolic engineering. RNA-based tools to control ribosome-dependent synthesis of proteins within E. coli were developed. First, adaptation of the well-studied RNA-IN and RNA-OUT system based on E. coli Tn10 to reliably repress translation regardless of coding sequence context was performed. Using an invariant sequence within the RNA-IN/OUT interacting region does not interfere with the function of the RNAs involved. The same RNA-IN/RNA-OUT system was adapted to activate the translation of a specific target mRNA. Regulation of translation extends to processing of polyproteins. In eukaryotes this can be accomplished by viral 2A peptides, avoiding reinitiating at a downstream start codon but has not been observed in bacteria. Therefore, a strategy for developing libraries of 2A peptides with potential activity across an array of organisms was developed."],"dc:identifier":["hdl:10133/5456"],"dc:subject":["2A-peptides","biochemistry","gene expression regulation","novel trans-acting RNAs","prokaryotic gene expression","synthetic biology","Dissertations, Academic"],"dc:title":["Development of RNA-based translation modulation tools for synthetic biology applications"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T20:02:20Z"}