{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/5247"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/5247","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"Distinguishing features of guide and substrate RNA recognition by H/ACA snoRNPs","abstract":"H/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs.","abstract_html":"H/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs.","abstract_has_math":false,"creators":["Kelly, Erin Katelyn","University of Lethbridge. Faculty of Arts and Science"],"institution":"Lethbridge, Alta. : Universtiy of Lethbridge, Department of Chemistry and Biochemistry","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Wieden-Kothe, Ute"],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018","date_published":"2018","updated_at":"2026-08-21T16:45:55Z","subjects":["RNA modification","H/ACA snoRNP","Saccharomyces cerevisiae","guide RNA","substrate RNA","Pseudouridine","Nucleoproteins","Non-coding RNA","Molecular biology","Dissertations, Academic"],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/5247"],"render_values":[{"text":"hdl:10133/5247","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/10133/5247","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"source_record":{"url":"https://opus.uleth.ca/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Aopus.uleth.ca%3A10133%2F5247","prefix":"dim"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.supervisor","label":"Supervisor","values":["Wieden-Kothe, Ute"]},{"key":"dc:creator","label":"Author","values":["Kelly, Erin Katelyn","University of Lethbridge. 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They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs."]},{"key":"dc:description.other","label":"Dc Description Other","values":["H/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs."]},{"key":"dc:title","label":"Title","values":["Distinguishing features of guide and substrate RNA recognition by H/ACA snoRNPs"]}]}],"canonical_facts":{"dc:contributor.supervisor":["Wieden-Kothe, Ute"],"dc:creator":["Kelly, Erin Katelyn","University of Lethbridge. Faculty of Arts and Science"],"dc:date.accessioned":["2018-11-26T17:49:17Z"],"dc:date.available":["2018-11-26T17:49:17Z"],"dc:date.issued":["2018"],"dc:description.abstract":["H/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs."],"dc:description.other":["H/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs."],"dc:identifier":["hdl:10133/5247"],"dc:identifier.uri":["https://hdl.handle.net/10133/5247"],"dc:language.iso":["en_US"],"dc:publisher":["Lethbridge, Alta. : Universtiy of Lethbridge, Department of Chemistry and Biochemistry"],"dc:publisher.department":["Department of Chemistry and Biochemistry"],"dc:subject":["RNA modification","H/ACA snoRNP","Saccharomyces cerevisiae","guide RNA","substrate RNA","Pseudouridine","Nucleoproteins","Non-coding RNA","Molecular biology","Dissertations, Academic"],"dc:title":["Distinguishing features of guide and substrate RNA recognition by H/ACA snoRNPs"],"dc:type":["Thesis"]},"updated_at":"2026-08-21T16:45:55Z"}