{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/5247"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/5247","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"Distinguishing features of guide and substrate RNA recognition by H/ACA snoRNPs","abstract":"H/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs.","abstract_html":"H/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs.","abstract_has_math":false,"creators":["Kelly, Erin Katelyn","University of Lethbridge. Faculty of Arts and Science"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018","date_published":"2018","updated_at":"2026-07-27T20:02:05Z","subjects":["RNA modification","H/ACA snoRNP","Saccharomyces cerevisiae","guide RNA","substrate RNA","Pseudouridine","Nucleoproteins","Non-coding RNA","Molecular biology","Dissertations, Academic"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/5247"],"render_values":[{"text":"hdl:10133/5247","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2018"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["RNA modification","H/ACA snoRNP","Saccharomyces cerevisiae","guide RNA","substrate RNA","Pseudouridine","Nucleoproteins","Non-coding RNA","Molecular biology","Dissertations, Academic"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/5247"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.other","label":"Dc Description Other","values":["H/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs."]},{"key":"dc:title","label":"Title","values":["Distinguishing features of guide and substrate RNA recognition by H/ACA snoRNPs"]}]}],"canonical_facts":{"dc:date.issued":["2018"],"dc:description.other":["H/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs."],"dc:identifier":["hdl:10133/5247"],"dc:subject":["RNA modification","H/ACA snoRNP","Saccharomyces cerevisiae","guide RNA","substrate RNA","Pseudouridine","Nucleoproteins","Non-coding RNA","Molecular biology","Dissertations, Academic"],"dc:title":["Distinguishing features of guide and substrate RNA recognition by H/ACA snoRNPs"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T20:02:05Z"}