{"id":{"repo_id":"lethbridge","oai_identifier":"oai:opus.uleth.ca:10133/4754"},"canonical_url":"https://search.dev.ndltd.org/etd/lethbridge/oai:opus.uleth.ca:10133/4754","repository":{"repo_id":"lethbridge","name":"University of Lethbridge","base_url":"https://opus.uleth.ca/server/oai/request"},"display":{"title":"Effects of extreme temperatures on the survival of the quarantine stored-product pest, Trogoderma granarium (khapra beetle) and on its associated bacteria","abstract":"Trogoderma granarium is a pest of stored-grain products in Asia and Africa, and a quarantine pest for much of the rest of the world. To evaluate extreme temperatures as a control strategy for this pest, I investigated the effect of high and low temperatures on the survival (Chapters 2 and 3) and on the microbiome (Chapter 4) of T. granarium. The most cold- and heat-tolerant life stages were diapausing-acclimated larvae and diapausing larvae, respectively. Trogoderma granarium can be controlled (Probit 9) with an exposure of 70 d to −15°C or 1.2 h to 60°C. High temperatures affected the microbiome; an effect of low temperatures was not detected. While the microbiome changed with life stage, it was dominated by Spiroplasma bacteria. Further research is necessary to understand the Spiroplasma-T. granarium relationship. Future research should also investigate combinations of extreme temperatures with other techniques to shorten the time required for mortality.","abstract_html":"Trogoderma granarium is a pest of stored-grain products in Asia and Africa, and a quarantine pest for much of the rest of the world. To evaluate extreme temperatures as a control strategy for this pest, I investigated the effect of high and low temperatures on the survival (Chapters 2 and 3) and on the microbiome (Chapter 4) of T. granarium. The most cold- and heat-tolerant life stages were diapausing-acclimated larvae and diapausing larvae, respectively. Trogoderma granarium can be controlled (Probit 9) with an exposure of 70 d to −15°C or 1.2 h to 60°C. High temperatures affected the microbiome; an effect of low temperatures was not detected. While the microbiome changed with life stage, it was dominated by Spiroplasma bacteria. Further research is necessary to understand the Spiroplasma-T. granarium relationship. Future research should also investigate combinations of extreme temperatures with other techniques to shorten the time required for mortality.","abstract_has_math":false,"creators":["Wilches Correal, Diana Maria","University of Lethbridge. Faculty of Arts and Science"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016","date_published":"2016","updated_at":"2026-07-27T20:02:20Z","subjects":["extreme temperature testing","insect microbiomes","insect pests","pest control methods","stored grain","stored-product pests"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/4754"],"render_values":[{"text":"hdl:10133/4754","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2016"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["extreme temperature testing","insect microbiomes","insect pests","pest control methods","stored grain","stored-product pests"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["hdl:10133/4754"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.other","label":"Dc Description Other","values":["Trogoderma granarium is a pest of stored-grain products in Asia and Africa, and a quarantine pest for much of the rest of the world. To evaluate extreme temperatures as a control strategy for this pest, I investigated the effect of high and low temperatures on the survival (Chapters 2 and 3) and on the microbiome (Chapter 4) of T. granarium. The most cold- and heat-tolerant life stages were diapausing-acclimated larvae and diapausing larvae, respectively. Trogoderma granarium can be controlled (Probit 9) with an exposure of 70 d to −15°C or 1.2 h to 60°C. High temperatures affected the microbiome; an effect of low temperatures was not detected. While the microbiome changed with life stage, it was dominated by Spiroplasma bacteria. Further research is necessary to understand the Spiroplasma-T. granarium relationship. Future research should also investigate combinations of extreme temperatures with other techniques to shorten the time required for mortality."]},{"key":"dc:title","label":"Title","values":["Effects of extreme temperatures on the survival of the quarantine stored-product pest, Trogoderma granarium (khapra beetle) and on its associated bacteria"]}]}],"canonical_facts":{"dc:date.issued":["2016"],"dc:description.other":["Trogoderma granarium is a pest of stored-grain products in Asia and Africa, and a quarantine pest for much of the rest of the world. To evaluate extreme temperatures as a control strategy for this pest, I investigated the effect of high and low temperatures on the survival (Chapters 2 and 3) and on the microbiome (Chapter 4) of T. granarium. The most cold- and heat-tolerant life stages were diapausing-acclimated larvae and diapausing larvae, respectively. Trogoderma granarium can be controlled (Probit 9) with an exposure of 70 d to −15°C or 1.2 h to 60°C. High temperatures affected the microbiome; an effect of low temperatures was not detected. While the microbiome changed with life stage, it was dominated by Spiroplasma bacteria. Further research is necessary to understand the Spiroplasma-T. granarium relationship. Future research should also investigate combinations of extreme temperatures with other techniques to shorten the time required for mortality."],"dc:identifier":["hdl:10133/4754"],"dc:subject":["extreme temperature testing","insect microbiomes","insect pests","pest control methods","stored grain","stored-product pests"],"dc:title":["Effects of extreme temperatures on the survival of the quarantine stored-product pest, Trogoderma granarium (khapra beetle) and on its associated bacteria"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T20:02:20Z"}