Abstract
In this work we studied various aspects of DNA repair in plants, focusing mainly on point mutation repair and its interconnection with double-strand break repair. We were using transgenic point mutation and recombination substrates as a primary tool in our experiments. We have compared two transgenic homologous recombination assays (B-glucuronidase- and luciferase-based), analyzed the sensitivity of DNA repair machinery to ultraviolet radiation and assessed the involvement of AtKu80, Atm and AtXpd repair genes in point mutation repair. Ours study revealed the following: the luciferase-based recombination assay is more sensitive then B-glucuronidase-based; double-stand break repair machinery is sensitive to ultraviolet radiation, which results in increased pint mutation formation; chosen DNA repair genes might be impaired in point mutation repair, however further experimentations are needed to confirm this.
Author and committee
dc:creator, dc:contributor.*- Authors
-
- Ilnytskyy, Yaroslav
- University of Lethbridge. Faculty of Arts and Science
Subjects
dc:subject × 4Identifiers
dc:identifier.*- Identifier
- hdl:10133/263
- OAI identifier oai:identifier
- oai:opus.uleth.ca:10133/263