{"id":{"repo_id":"laurentian","oai_identifier":"oai:laurentian.scholaris.ca:10219/2884"},"canonical_url":"https://search.dev.ndltd.org/etd/laurentian/oai:laurentian.scholaris.ca:10219/2884","repository":{"repo_id":"laurentian","name":"Laurentian University","base_url":"https://laurentian.scholaris.ca/server/oai/request"},"display":{"title":"Correlation between phenotypes or genotypes of Clostridium difficile and clinical outcome from Clostridium difficile infections at Health Sciences North","abstract":"Clostridium difficile, a Gram-positive, anaerobic, spore producing bacillus is the leading cause of nosocomial diarrhea in health-care facilities. Its effect on patients can be detrimental to their recovery, sometimes leading to death; the catalyst to Clostridium difficile infection (CDI) is treatment by antibiotics. Focusing research on some of C. difficile’s genotypes and phenotypes, we hoped to be able to predict the severity and outcome of the infections based on the strain. C. difficile strains isolated from patients were characterized for the presence of the toxin genes and classified by ribotype. They were also tested for antibiotic susceptibility (E-test), toxin production levels, and microbial spore load in stools. The patients symptoms were evaluated to determine if there were any correlations between the genotypes/phenotypes and the infection outcomes. No significant correlation was found between the severity of disease and phenotypic/genotypic attributes of the C. difficile strain involved. Thus, other defining factors appear to affect patient prognosis such as the severity of the patients’ disease and the presence of co-morbidities. A key finding however was that 30% of the population was at risk of developing CDI due to carriage of toxigenic C. difficile.","abstract_html":"Clostridium difficile, a Gram-positive, anaerobic, spore producing bacillus is the leading cause of nosocomial diarrhea in health-care facilities. Its effect on patients can be detrimental to their recovery, sometimes leading to death; the catalyst to Clostridium difficile infection (CDI) is treatment by antibiotics. Focusing research on some of C. difficile’s genotypes and phenotypes, we hoped to be able to predict the severity and outcome of the infections based on the strain. C. difficile strains isolated from patients were characterized for the presence of the toxin genes and classified by ribotype. They were also tested for antibiotic susceptibility (E-test), toxin production levels, and microbial spore load in stools. The patients symptoms were evaluated to determine if there were any correlations between the genotypes/phenotypes and the infection outcomes. No significant correlation was found between the severity of disease and phenotypic/genotypic attributes of the C. difficile strain involved. Thus, other defining factors appear to affect patient prognosis such as the severity of the patients’ disease and the presence of co-morbidities. A key finding however was that 30% of the population was at risk of developing CDI due to carriage of toxigenic C. difficile.","abstract_has_math":false,"creators":["Lefebvre, Sebastien Roger"],"institution":"Laurentian University of Sudbury","degree_name":"Master of Science (MSc) in Biology","degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-01-26","date_published":"2016-01-26","updated_at":"2026-08-21T16:45:57Z","subjects":["Antibiotic resistance","antibiotic susceptibility","Clostridium difficile","genotype phenotype","ribotype","spores","TcdA","TcdB","toxin"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://laurentian.scholaris.ca/handle/10219/2884","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"source_record":{"url":"https://laurentian.scholaris.ca/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Alaurentian.scholaris.ca%3A10219%2F2884","prefix":"dim"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Lefebvre, Sebastien Roger"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-02-06T16:29:10Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-02-06T16:29:10Z"]},{"key":"dc:date.issued","label":"Date","values":["2016-01-26"]},{"key":"dc:publisher","label":"Institution","values":["Laurentian University of Sudbury"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MSc) in Biology"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Laurentian University of Sudbury"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Antibiotic resistance","antibiotic susceptibility","Clostridium difficile","genotype phenotype","ribotype","spores","TcdA","TcdB","toxin"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://laurentian.scholaris.ca/handle/10219/2884"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Clostridium difficile, a Gram-positive, anaerobic, spore producing bacillus is the leading cause of nosocomial diarrhea in health-care facilities. Its effect on patients can be detrimental to their recovery, sometimes leading to death; the catalyst to Clostridium difficile infection (CDI) is treatment by antibiotics. Focusing research on some of C. difficile’s genotypes and phenotypes, we hoped to be able to predict the severity and outcome of the infections based on the strain. C. difficile strains isolated from patients were characterized for the presence of the toxin genes and classified by ribotype. They were also tested for antibiotic susceptibility (E-test), toxin production levels, and microbial spore load in stools. The patients symptoms were evaluated to determine if there were any correlations between the genotypes/phenotypes and the infection outcomes. No significant correlation was found between the severity of disease and phenotypic/genotypic attributes of the C. difficile strain involved. Thus, other defining factors appear to affect patient prognosis such as the severity of the patients’ disease and the presence of co-morbidities. A key finding however was that 30% of the population was at risk of developing CDI due to carriage of toxigenic C. difficile."]},{"key":"dc:title","label":"Title","values":["Correlation between phenotypes or genotypes of Clostridium difficile and clinical outcome from Clostridium difficile infections at Health Sciences North"]}]}],"canonical_facts":{"dc:creator":["Lefebvre, Sebastien Roger"],"dc:date.accessioned":["2018-02-06T16:29:10Z"],"dc:date.available":["2018-02-06T16:29:10Z"],"dc:date.issued":["2016-01-26"],"dc:description.abstract":["Clostridium difficile, a Gram-positive, anaerobic, spore producing bacillus is the leading cause of nosocomial diarrhea in health-care facilities. Its effect on patients can be detrimental to their recovery, sometimes leading to death; the catalyst to Clostridium difficile infection (CDI) is treatment by antibiotics. Focusing research on some of C. difficile’s genotypes and phenotypes, we hoped to be able to predict the severity and outcome of the infections based on the strain. C. difficile strains isolated from patients were characterized for the presence of the toxin genes and classified by ribotype. They were also tested for antibiotic susceptibility (E-test), toxin production levels, and microbial spore load in stools. The patients symptoms were evaluated to determine if there were any correlations between the genotypes/phenotypes and the infection outcomes. No significant correlation was found between the severity of disease and phenotypic/genotypic attributes of the C. difficile strain involved. Thus, other defining factors appear to affect patient prognosis such as the severity of the patients’ disease and the presence of co-morbidities. A key finding however was that 30% of the population was at risk of developing CDI due to carriage of toxigenic C. difficile."],"dc:identifier.uri":["https://laurentian.scholaris.ca/handle/10219/2884"],"dc:language.iso":["en"],"dc:publisher":["Laurentian University of Sudbury"],"dc:subject":["Antibiotic resistance","antibiotic susceptibility","Clostridium difficile","genotype phenotype","ribotype","spores","TcdA","TcdB","toxin"],"dc:title":["Correlation between phenotypes or genotypes of Clostridium difficile and clinical outcome from Clostridium difficile infections at Health Sciences North"],"dc:type":["Thesis"],"thesis:degree_name":["Master of Science (MSc) in Biology"],"thesis:institution_name":["Laurentian University of Sudbury"]},"updated_at":"2026-08-21T16:45:57Z"}