{"id":{"repo_id":"laurentian","oai_identifier":"oai:laurentian.scholaris.ca:10219/2841"},"canonical_url":"https://search.dev.ndltd.org/etd/laurentian/oai:laurentian.scholaris.ca:10219/2841","repository":{"repo_id":"laurentian","name":"Laurentian University","base_url":"https://laurentian.scholaris.ca/server/oai/request"},"display":{"title":"Examination of gene deletion as a mechanism of RBM5 downregulation in tobacco smoke-associated lung cancer","abstract":"RBM5 is a tumour suppressor gene with substantially decreased expression in most lung cancers, especially in smokers, that may result from gene deletion. The objective of this study was to determine if significantly decreased levels of RBM5 expression in the lung cancers of smokers was related to RBM5 deletion. Using DNA from patient lung tissue, RBM5 gene copy number was quantified by quantitative polymerase chain reaction. Although deletions were detected in the lung specimens, RBM5 gene copy number was not significantly decreased in lung tumours. RBM5 deletions were detected in the non-tumour DNA of smokers, but not in the never-smokers, indicating that RBM5 gene deletion might be related to smoking. In conclusion, it appeared as though RBM5 was downregulated by more than one mechanism, which included gene deletion. Further analyses must be carried out to examine other mechanisms by which RBM5 is downregulated.","abstract_html":"RBM5 is a tumour suppressor gene with substantially decreased expression in most lung cancers, especially in smokers, that may result from gene deletion. The objective of this study was to determine if significantly decreased levels of RBM5 expression in the lung cancers of smokers was related to RBM5 deletion. Using DNA from patient lung tissue, RBM5 gene copy number was quantified by quantitative polymerase chain reaction. Although deletions were detected in the lung specimens, RBM5 gene copy number was not significantly decreased in lung tumours. RBM5 deletions were detected in the non-tumour DNA of smokers, but not in the never-smokers, indicating that RBM5 gene deletion might be related to smoking. In conclusion, it appeared as though RBM5 was downregulated by more than one mechanism, which included gene deletion. Further analyses must be carried out to examine other mechanisms by which RBM5 is downregulated.","abstract_has_math":false,"creators":["Hunt, Sarah C."],"institution":"Laurentian University of Sudbury","degree_name":"Master of Science (MSc) in Chemical Sciences","degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-10-13","date_published":"2017-10-13","updated_at":"2026-08-21T16:45:55Z","subjects":["RBM5","non-small cell lung cancer","gene copy number","gene deletion","expression","tobacco smoke exposure","smokers","never-smokers"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://laurentian.scholaris.ca/handle/10219/2841","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"source_record":{"url":"https://laurentian.scholaris.ca/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Alaurentian.scholaris.ca%3A10219%2F2841","prefix":"dim"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Hunt, Sarah C."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-11-16T14:26:42Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-11-16T14:26:42Z"]},{"key":"dc:date.issued","label":"Date","values":["2017-10-13"]},{"key":"dc:publisher","label":"Institution","values":["Laurentian University of Sudbury"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MSc) in Chemical Sciences"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Laurentian University of Sudbury"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["RBM5","non-small cell lung cancer","gene copy number","gene deletion","expression","tobacco smoke exposure","smokers","never-smokers"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://laurentian.scholaris.ca/handle/10219/2841"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["RBM5 is a tumour suppressor gene with substantially decreased expression in most lung cancers, especially in smokers, that may result from gene deletion. The objective of this study was to determine if significantly decreased levels of RBM5 expression in the lung cancers of smokers was related to RBM5 deletion. Using DNA from patient lung tissue, RBM5 gene copy number was quantified by quantitative polymerase chain reaction. Although deletions were detected in the lung specimens, RBM5 gene copy number was not significantly decreased in lung tumours. RBM5 deletions were detected in the non-tumour DNA of smokers, but not in the never-smokers, indicating that RBM5 gene deletion might be related to smoking. In conclusion, it appeared as though RBM5 was downregulated by more than one mechanism, which included gene deletion. Further analyses must be carried out to examine other mechanisms by which RBM5 is downregulated."]},{"key":"dc:title","label":"Title","values":["Examination of gene deletion as a mechanism of RBM5 downregulation in tobacco smoke-associated lung cancer"]}]}],"canonical_facts":{"dc:creator":["Hunt, Sarah C."],"dc:date.accessioned":["2017-11-16T14:26:42Z"],"dc:date.available":["2017-11-16T14:26:42Z"],"dc:date.issued":["2017-10-13"],"dc:description.abstract":["RBM5 is a tumour suppressor gene with substantially decreased expression in most lung cancers, especially in smokers, that may result from gene deletion. The objective of this study was to determine if significantly decreased levels of RBM5 expression in the lung cancers of smokers was related to RBM5 deletion. Using DNA from patient lung tissue, RBM5 gene copy number was quantified by quantitative polymerase chain reaction. Although deletions were detected in the lung specimens, RBM5 gene copy number was not significantly decreased in lung tumours. RBM5 deletions were detected in the non-tumour DNA of smokers, but not in the never-smokers, indicating that RBM5 gene deletion might be related to smoking. In conclusion, it appeared as though RBM5 was downregulated by more than one mechanism, which included gene deletion. Further analyses must be carried out to examine other mechanisms by which RBM5 is downregulated."],"dc:identifier.uri":["https://laurentian.scholaris.ca/handle/10219/2841"],"dc:language.iso":["en"],"dc:publisher":["Laurentian University of Sudbury"],"dc:subject":["RBM5","non-small cell lung cancer","gene copy number","gene deletion","expression","tobacco smoke exposure","smokers","never-smokers"],"dc:title":["Examination of gene deletion as a mechanism of RBM5 downregulation in tobacco smoke-associated lung cancer"],"dc:type":["Thesis"],"thesis:degree_name":["Master of Science (MSc) in Chemical Sciences"],"thesis:institution_name":["Laurentian University of Sudbury"]},"updated_at":"2026-08-21T16:45:55Z"}